Predominance of lineage I among Trypanosoma cruzi isolates from Venezuelan patients with different clinical profiles of acute Chagas' disease
Predominance of lineage I among Trypanosoma cruzi isolates from Venezuelan patients with different clinical profiles of acute Chagas' disease
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DOI：
10.1111/j.1365-3156.2004.01333.x
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发表时间：
2004-12-01
影响因子：
3.3
通讯作者：
Teixeira, MMG
中科院分区：
文献类型：
--
作者：
Añez, N;Crisante, G;Teixeira, MMG
利用核糖体和小外显子基因标记对委内瑞拉西部（巴里纳斯州）恰加斯病流行地区23例急性恰加斯病患者分离的克氏锥虫进行分型。结果表明，从这些患者中分离到克氏锥虫I型和克氏锥虫II型两个主要系统发育谱系。6株（26%）被分型为克氏锥虫II型，17株（74%）被分型为克氏锥虫i型。随机扩增多态性DNA （RAPD）分析证实了这两组分离株，但未发现明显的遗传谱系内多态性。感染克鲁兹锥虫I型或克鲁兹锥虫II型的患者表现出不同的临床特征，表现出恰加斯病急性期从完全无症状到严重心力衰竭的高度变化的体征和症状。在委内瑞拉，克氏体1型人类分离株的优势，以及该谱系感染患者出现严重恰加斯病（心力衰竭）症状的较高流行率，不能证实克氏体1型感染对人类无害。据我们所知，这是第一个在大量急性恰加斯病患者中描述克氏锥虫I系优势的研究，这些患者具有明显的临床特征。
Trypanosoma cruzi isolates from 23 acute chagasic patients from localities of Western Venezuela (state of Barinas) where Chagas' disease is endemic were typed using ribosomal and mini-exon gene markers. Results showed that isolates of the two major phylogenetic lineages, T. cruzi I and T. cruzi II, were isolated from these patients. Six isolates (26%) were typed as T. cruzi II and 17 (74%) as belonging to T. cruzi lineage I. Analysis of random amplified polymorphic DNA (RAPD) patterns confirmed these two groups of isolates, but did not disclose significant genetic intra-lineage polymorphism. Patients infected by both T. cruzi I or T. cruzi II showed different clinical profiles presenting highly variable signs and symptoms of acute phase of Chagas' disease ranging from totally asymptomatic to severe heart failure. The predominance of T. cruzi I human isolates in Venezuela allied to the higher prevalence of severe symptoms of Chagas' disease (heart failure) in patients infected by this lineage do not corroborate an innocuousness of T. cruzi I infection to humans. To our knowledge, this is the first study describing predominance of T. cruzi lineage I in a large number of acute chagasic patients with distinct and well-characterized clinical profiles.