Perifused pituitary cultures: a model for LHRH regulation of LH secretion.
Perifused pituitary cultures: a model for LHRH regulation of LH secretion.
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垂体灌注培养物：LHRH 调节 LH 分泌的模型。
DOI：
10.1152/ajpendo.1981.240.6.e591
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
CrowleyJr,WF
中科院分区：
文献类型：
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作者：
Loughlin,JS;Badger,TM;CrowleyJr,WF
本文介绍了一种适合于黄体生成素释放激素(LHRH)-垂体前叶生理学研究的分散的、连续灌流的垂体前叶细胞系统。成年雄性SD大鼠断头，摘除垂体前叶，胶原酶分散，与生物凝胶P2混合，用无菌技术包装成0.9 cm长的柱子。所有实验均使用Krebs-Ringer-碳酸氢盐-葡萄糖缓冲液进行，并获得了稳定的基线。恒定剂量的LHRH(50 Ng)，每隔1小时多次给药，可刺激同样数量的黄体生成素的释放。促黄体生成素的分泌在1-50 NM范围内与LHRH呈线性关系，与大鼠下丘脑提取物在1/32-1/2相当于下丘脑的范围内呈线性关系，无论提取物是按升序还是降序给药。这些结果表明，分散的垂体细胞灌流结合了垂体细胞在培养中长期存活的优势和灌流所提供的动态特性，为LHRH生理学的研究提供了一个有用的模型。
In this paper, a dispersed anterior pituitary cell system that is continuously perifused and suitable for the study of luteinizing hormone-releasing hormone (LHRH)-anterior pituitary physiology is described. Adult male Sprague-Dawley rats were decapitated and the anterior pituitaries removed, dispersed using collagenase, mixed with Biogel P2, and packed into 0.9-cm columns using sterile technique. All experiments were conducted using Krebs-Ringer-bicarbonate-glucose buffer, and a stable base line was achieved. A constant dose of LHRH (50 ng) administered several times at 1-h intervals stimulated the same quantal release of LH. LH secretion is linear in response to LHRH over the range of 1-50 nM and to rat hypothalamic extracts over a range of 1/32 to 1/2 hypothalamic equivalents, regardless of whether the extracts are administered in ascending or descending order. These results indicate that perifusion of dispersed pituitary cells provides a useful model for the study of LHRH physiology, combining the advantages of the long-term viability of pituitary cells in culture with the dynamic nature afforded by perifusion.