A filter paper coated with phenylboronic acid-modified mesoporous silica for enrichment of intracellular nucleosides prior to their quantitation by HPLC
A filter paper coated with phenylboronic acid-modified mesoporous silica for enrichment of intracellular nucleosides prior to their quantitation by HPLC
复制标题
涂有苯基硼酸改性介孔二氧化硅的滤纸，用于在 HPLC 定量之前富集细胞内核苷
DOI：
10.1007/s00604-017-2423-9
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发表时间：
2017-10-01
影响因子：
5.7
通讯作者：
Zhang, Haixia
中科院分区：
文献类型：
--
作者：
Cheng, Ting;Zhang, Yuan;Zhang, Haixia
作者描述了一种用介孔二氧化硅改性的滤纸，其表面携带苯基硼酸。该材料具有滤纸的柔韧性和介孔二氧化硅的大比表面积的优点。这使其成为富集痕量核苷的优良吸附剂。检测限（在HPLC分析与UV检测中）为2.0-11.1 μ g L-1，定量限为6.8-37.1 μ g L-1，具有良好的精密度（RSD < 6.4%）。与普通滤纸和无孔二氧化硅涂层滤纸相比，该材料具有更高的效率和4.5倍的容量。通过从HepG 2细胞中预浓缩核苷来证明潜在的应用，HepG 2细胞是代表性的复杂样品。该方法也被用来研究30纳米金纳米粒子对细胞代谢的影响。
The authors describe a filter paper modified with mesoporous silica that carries phenylboronic acid on its surface. The material possesses the advantages of the flexibility of filter paper and the large surface of mesoporous silica. This makes it an excellent sorbent for the enrichment of trace quantities of nucleosides. The limits of detection (in HPLC analysis with UV detection) are 2.0-11.1 mu g L-1 and the limits of quantification are 6.8-37.1 mu g L-1 with good precision (RSD < 6.4%). Compared to plain filter paper and nonporous silica-coated filter paper, the material exhibits enhanced efficiency and a 4.5-fold improved capacity. The potential applications are demonstrated by preconcentrating nucleosides from HepG2 cells which are a representative complex sample. The method was also used to study the effect of 30-nm gold nanoparticle on cellular metabolism.