PEG-PLA Nanocapsules Containing a Nanobiotechnological Complex of Polyhemoglobin-Tyrosinase for the Depletion of Tyrosine in Melanoma: Preparation and In Vitro Characterisation.
PEG-PLA Nanocapsules Containing a Nanobiotechnological Complex of Polyhemoglobin-Tyrosinase for the Depletion of Tyrosine in Melanoma: Preparation and In Vitro Characterisation.
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含有多聚血红蛋白-酪氨酸酶纳米生物技术复合物的 PEG-PLA 纳米胶囊，用于消耗黑色素瘤中的酪氨酸：制备和体外表征。
DOI：
10.4172/2155-983x.1000103
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
T. Chang
中科院分区：
文献类型：
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作者：
Caroline Fustier;T. Chang
对聚乙二醇-聚乳酸嵌段共聚物（PEG-PLA）进行了结构优化，并用核磁共振氢谱和差示扫描量热法（DSC）对PEG-PLA进行了表征。然后将其用于制备含有聚血红蛋白-酪氨酸酶的PEG-PLA纳米胶囊。透射电子显微镜和扫描电子显微镜的研究表明，圆形和非聚集的纳米胶囊与PEG晕周围的每个纳米胶囊。动态光散射显示Z-平均直径为65.2 ± 0.5 nm（平均值± SEM），多分散指数为0.262 ± 0.002。控制直径的因素包括反应混合物的搅拌速度和PEG-PLA共聚物中PLA嵌段的尺寸。在体温37 ℃时，8小时后游离酪氨酸酶失去全部酶活性。然而，聚血红蛋白-酪氨酸酶纳米囊在8小时后保留其初始活性的80%。本论文的第一部分工作是聚乙二醇聚乳酸聚血红蛋白酪氨酸酶纳米胶囊的制备和体外表征。在大鼠中的初步结果显示，1次静脉注射在5分钟后将全身酪氨酸水平降低至10-13%。详细的体外研究和初步的动物研究的结果导致我们正在进行的详细的动物研究将在随后的论文中报告。
Poly (ethylene glycol)-poly (lactic acid) block-copolymer (PEG-PLA) was optimized and characterized using H-NMR spectrum and DSC thermogram. This was then used for the preparation of PEG-PLA nanocapsules containing polyhemoglobin-tyrosinase. Transmission electron microscopic and scanning electron microscopic studies showed round and non-aggregated nanocapsules with a PEG halo around each nanocapsule. Dynamic Light Scattering showed that the Z-average diameter was 65.2 ± 0.5 nm (mean ± SEM) and the polydispersity index was 0.262 ± 0.002. Factors controlling the diameters included the stirring speed of the reaction mixture and the size of the PLA block in the PEG-PLA copolymer. At the body temperature of 37oC, free tyrosinase lost all its enzyme activity after 8 hours. However, Polyhemoglobin-tyrosinase nanocapcules retained 80% of its initial activity after 8 hours. This paper contains the first part of our work on the preparation and in vitro characterisation of PEG-PLA Polyhemoglobin-tyrosinase nanocapsules. Preliminary result in rats shows that 1 intravenous injection lowers the systemic tyrosine level to 10-13% after 5 minutes. The result of the detailed in vitro study and the preliminary animal study in have led to our ongoing detailed animal research to be reported in subsequent papers.