Group I mGlu receptors regulate the expression of the neuronal calcium sensor protein VILIP-1 in vitro and in vivo: implications for mGlu receptor-dependent hippocampal plasticity?
Group I mGlu receptors regulate the expression of the neuronal calcium sensor protein VILIP-1 in vitro and in vivo: implications for mGlu receptor-dependent hippocampal plasticity?
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DOI：
10.1016/s0028-3908(03)00051-0
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发表时间：
2003-05-01
影响因子：
4.7
通讯作者：
Manahan-Vaughan, D
中科院分区：
文献类型：
--
作者：
Braunewell, KH;Brackmann, M;Manahan-Vaughan, D
代谢型谷氨酸 (mGlu) 受体参与大鼠海马体中多种形式的突触可塑性。激活 I 组 mGlu 受体的激动剂诱导海马区 CA1 中缓慢起效的增强作用，而无需事先强直作用。 I 组 mGlu 受体的激活诱导蛋白质合成，这可能有助于 mGlu 受体依赖性形式的长期可塑性。钙结合蛋白被广泛认为是突触可塑性的关键元素。因此，我们研究了钙传感蛋白 VILIP-I 是否与体内齿状回 (DG) 中 I 组 mGlu 受体介导的可塑性相关。应用 I 组和 II 组 mGlu 激动剂 (1S,3R)-1-氨基环戊烷-1,3-二羧酸酯 (ACPD) 或选择性 I 组激动剂 (R,S)-3,5-二羟基苯基甘氨酸 (DHPG) 导致缓慢起病成年大鼠 DG 的增强作用。在海马细胞培养物中，两种激动剂均引起 VILIP-1 表达增强。原位杂交显示 VILIP-1 的海马表达强烈，成年大鼠脑内注射 DHPG 显着增强了海马 VILIP-1 的表达。 I 组 mGlu 受体拮抗剂 4-羧基-苯基甘氨酸 (4CPG) 可以阻止 DHPG 在海马培养物和体内的作用。因此，钙传感器蛋白似乎以活性依赖性方式受到 mGlu 受体的调节。 I 组 mGlu 受体的特殊作用是显而易见的。此外，传感器蛋白可能充当长期调节突触可塑性的分子开关。 (C) 2003 Elsevier Science Ltd. 保留所有权利。
Metabotropic glutamate (mGlu) receptors are involved in several forms of synaptic plasticity in the rat hippocampus. Agonists which activate group I mGlu receptors induce slow-onset potentiation without prior tetanization in the hippocampal area CA1. Activation of group I mGlu receptors induces protein synthesis which may contribute to mGlu receptor-dependent forms of long-term plasticity. Calcium-binding proteins are widely considered to comprise key elements for synaptic plasticity. Therefore, we investigated whether the calcium sensor protein VILIP-I is associated with group I mGlu receptor-mediated plasticity in the dentate gyrus (DG) in vivo.Application of either the group I and II mGlu agonist (1S,3R)-1-aminocyclopentane-1,3-dicarboxylate (ACPD) or the selective group I agonist (R,S)-3,5-dihydroxyphenylglycine (DHPG) resulted in slow-onset potentiation in the DG of adult rats. In hippocampal cell cultures both agonists elicited an enhanced expression of VILIP-1. In situ hybridization revealed strong hippocampal expression of VILIP-I and intracerebral application of DHPG to adult rats significantly enhanced hippocampal VILIP-1 expression. The DHPG effects in both, hippocampal cultures and in vivo, were prevented by the group I mGlu receptor antagonist 4-Carboxy-phenylglycine (4CPG).Calcium sensor proteins thus appear to be regulated by mGlu receptors in an activity-dependent manner. A specific role for group I mGlu receptors is evident. Furthermore, the sensor proteins may function as molecular switches for the long-term regulation of synaptic plasticity. (C) 2003 Elsevier Science Ltd. All rights reserved.