Immunohistochemical localization of neurocalcin in human sensory neurons and mechanoreceptors
Immunohistochemical localization of neurocalcin in human sensory neurons and mechanoreceptors
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DOI：
10.1016/s0304-3940(99)00950-7
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发表时间：
2000-01-28
影响因子：
2.5
通讯作者：
Vega, JA
中科院分区：
文献类型：
--
作者：
Galeano, RM;Germanà, A;Vega, JA
用免疫组织化学方法研究了神经钙蛋白在发育和成年人周围神经系统（背根和交感神经节（DRG，SG）和肠神经系统（ENS））中的定位。9周龄和12周龄的胚胎DRG中的大型神经元的亚群显示免疫反应性（IR），而交感神经节或肠神经元没有。在成人中，神经钙蛋白IR仅限于DRG中的大型（13%）和中型（15%）神经元的亚群。该蛋白质也被发现在肌肉（67%）和皮肤（12%）的神经纤维，以及在轴突供应肌肉（肌梭，高尔基氏腱器官，和perimysial Pacinian小体）和皮肤（迈斯纳的，但不是Pacinian小体）机械感受器，以及运动终板。目前的研究结果表明，神经钙蛋白在发展中国家和成年人可以被用作一个特定的标记物的感觉神经元的亚群耦合到本体感觉和触摸，和运动神经元形成运动终板的轴突。(C)2000爱思唯尔科学爱尔兰有限公司保留所有权利。
The localization of neurocalcin in the developing and adult human peripheral nervous system (dorsal root and sympathetic ganglia (DRG, SG), and enteric nervous system (ENS)) was investigated using immunohistochemistry. A subpopulation of large-sized neurons in DRG of 9 and 12 weeks old embryos showed immunoreactivity (IR), whereas the sympathetic ganglia or enteric neurons did not. In adults, neurocalcin IR was restricted to a subpopulation of large (13%) and intermediate (15%) sized neurons in DRG. The protein was also found in muscular (67%) and cutaneous (12%) nerve fibers, as well as in the axons supplying muscular (muscle spindles, Golgi's tendon organs, and perimysial Pacinian corpuscles) and cutaneous (Meissner's but not Pacinian corpuscles) mechanoreceptors, as well as motor end-plates. Present results demonstrate that neurocalcin in both developing and adult humans can be used as a specific marker for a subpopulation of sensory neurons coupled to proprioception and touch, and for axons of motoneurons forming motor end-plates. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.