Genetic diversity and intergenogroup recombination events of sapoviruses detected from feces of pigs in Japan
Genetic diversity and intergenogroup recombination events of sapoviruses detected from feces of pigs in Japan
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DOI：
10.1016/j.meegid.2017.09.013
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发表时间：
2017-11-01
影响因子：
3.2
通讯作者：
Nagai, Makoto
中科院分区：
文献类型：
--
作者：
Kuroda, Moegi;Masuda, Tsuneyuki;Nagai, Makoto
Sapoviruses（SaV）是感染人类和动物的肠道病毒。SaV具有高度多样性，并基于结构蛋白（VP1）序列分为多个基因组。从猪中检测到的SaV属于8个基因组（GIII、GV、GVI、GVII、GVIII、GIX、GX和GXI），但对SaV基因组在日本猪群体中的分布知之甚少。本研究通过宏基因组学方法从日本的1头嗜热猪和15头非嗜热猪粪便中获得了26个几乎完整的基因组（> 6000 nt）和3个部分序列（长度分别为2429 nt、4364 nt和4419 nt，包括整个VP1编码区）。系统发育分析表明，29株猪SaV可分为7个基因组：GIII（11株）、GV（1株）、GVI（3株）、GVII（6株）、GVIII（1株）、GX（3株）和GXI（4株）。这份手稿提出了第一个几乎完整的基因组序列的GX和GXI，并证明了新的基因组间重组事件。
Sapoviruses (SaV) are enteric viruses infecting humans and animals. SaVs are highly diverse and are divided into multiple genogroups based on structural protein (VP1) sequences. SaVs detected from pigs belong to eight genogroups (GIII, GV, GVI, GVII, GVIII, GIX, GX, and GXI), but little is known about the SaV genogroup distribution in the Japanese pig population. In the present study, 26 nearly complete genome (> 6000 nucleotide: nt) and three partial sequences (2429 nt, 4364 nt, and 4419 nt in length, including the entire VP1 coding region) of SaV were obtained from one diarrheic and 15 non-diarrheic porcine feces in Japan via a metagenomics approach. Phylogenetic analysis of the complete VP1 amino acid sequence (aa) revealed that 29 porcine SaVs were classified into seven genogroups; GIII (11 strains), GV (1 strain), GVI (3 strains), GVII (6 strains), GVIII (1 strain), GX (3 strains), and GXI (4 strains). This manuscript presents the first nearly complete genome sequences of GX and GXI, and demonstrates novel intergenogroup recombination events.