Renal expression of the gene encoding the gastric H(+)-K(+)-ATPase beta-subunit.
Renal expression of the gene encoding the gastric H(+)-K(+)-ATPase beta-subunit.
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编码胃 H( )-K( )-ATP 酶 β 亚基的基因的肾脏表达。
DOI：
10.1152/ajprenal.1995.268.3.f363
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Cain,BD
中科院分区：
文献类型：
--
作者：
Callaghan,JM;Tan,SS;Khan,MA;Curran,KA;Campbell,WG;Smolka,AJ;Toh,BH;Gleeson,PA;Wingo,CS;Cain,BD
胃粘膜壁细胞和肾集合管细胞均具有H(+)-K(+)-腺苷三磷酸酶(H(+)-K(+)-ATPase)活性。在胃中，H(+)-K(+)-ATPase(EC 3.6.1.3)负责腔内容物的酸化。肾脏H(+)-K(+)-ATPase蛋白(S)有助于钾的重吸收和氢离子的分泌，以维持钾和酸碱的动态平衡。胃H(+)-K(+)-ATPase由一个表观分子量为95 kDa的α-催化亚基和一个60-90 kDa的高度糖基化的β-亚基组成。本文讨论了调节肾脏H(+)-K(+)-ATPase活性的蛋白质的分子同一性。RNA杂交、肾脏RNA聚合酶链式反应和cDNA序列分析相结合的结果表明，肾脏中存在胃H(+)-K(+)-ATPaseβ亚基mRNA。用抗胃H(+)-K(+)-ATPaseβ亚基的抗体免疫印迹检测到的蛋白质，脱糖后与兔、猪、大鼠和小鼠肾脏膜蛋白制剂中的胃β亚基具有相同的分子质量。此外，我们使用转基因小鼠证明了胃H(+)-K(+)-ATPaseβ亚基基因包含顺式作用调控序列，该序列在胃壁细胞和肾脏集合管中都是活跃的。总之，这些数据表明，胃H(+)-K(+)-ATPaseβ亚基存在于肾脏中，可能与胃H(+)-K(+)-ATPaseα亚基和/或其他P型ATPaseα亚基有关，从而有助于酸碱和钾的动态平衡。
The gastric mucosal parietal cells and cells of the renal collecting duct both possess H(+)-K(+)-adenosinetriphosphatase (H(+)-K(+)-ATPase) activities. In the stomach, the H(+)-K(+)-ATPase (EC 3.6.1.3) is responsible for acidification of luminal contents. The kidney H(+)-K(+)-ATPase protein(s) contribute to potassium reabsorption and secretion of hydrogen ions to maintain potassium and acid-base homeostasis. The stomach H(+)-K(+)-ATPase is well defined and consists of an alpha-catalytic subunit of apparent molecular mass of 95 kDa and a highly glycosylated beta-subunit of 60-90 kDa. The molecular identity of the protein that mediates the H(+)-K(+)-ATPase activity in the kidney has been addressed in this paper. A combination of RNA hybridizations, polymerase chain reaction analysis of kidney RNA, and sequence analysis of cDNAs indicated that gastric H(+)-K(+)-ATPase beta-subunit mRNA is present in kidney. Immunoblotting with antibodies specific for the gastric H(+)-K(+)-ATPase beta-subunit detected proteins, which, after deglycosylation, had the same molecular mass as the gastric beta-subunit in membrane protein preparations from rabbit, pig, rat, and mouse kidneys. Furthermore, we have used transgenic mice to demonstrate that the gastric H(+)-K(+)-ATPase beta-subunit gene contains cis-acting regulatory sequences that are active in both gastric parietal cells and the renal collecting ducts. Overall, these data indicate that the gastric H(+)-K(+)-ATPase beta-subunit is found in the kidney and probably associates with the gastric H(+)-K(+)-ATPase alpha-subunit and/or other P-type ATPase alpha-subunits, thus contributing to acid-base and potassium homeostasis.