In vitro systems for tissue engineering
In vitro systems for tissue engineering
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DOI：
10.1111/j.1749-6632.2002.tb03041.x
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发表时间：
2002-01-01
期刊：
影响因子：
--
通讯作者：
Atala, A
中科院分区：
文献类型：
--
作者：
Godbey, WT;Atala, A
组织工程学，必然，包括广泛的实验方向和科学学科。体外组织工程涉及在植入体内环境之前在体外操作细胞。相比之下，体内组织工程依赖于身体的自然再生能力，而不是非细胞接种的生物材料。细胞、生物材料和可控的孵育条件在现代组织工程体外系统的构建和使用中都起着重要作用。在适当的情况下，基因递送也是控制或支持体外和植入后工程化细胞功能的重要因素。在这篇综述中，涉及在体外组织工程的背景下，包括生物反应器，细胞接种的构建体，细胞封装，和基因递送系统的地址。重点放在与临床条件的治疗更直接相关的研究上。
Tissue engineering, by necessity, encompasses a wide array of experimental directions and scientific disciplines. In vitro tissue engineering involves the manipulation of cells in vitro, prior to implantation into the in vivo environment. In contrast, in vivo tissue engineering relies on the body's natural ability to regenerate over non-cell-seeded biomaterials. Cells, biomaterials, and controlled incubation conditions all play important roles in the construction and use of modern in vitro systems for tissue engineering. Gene delivery is also an important factor for controlling or supporting the function of engineered cells both in vitro and post implantation, where appropriate. In this review, systems involved in the context of in vitro tissue engineering are addressed, including bioreactors, cell-seeded constructs, cell encapsulation, and gene delivery. Emphasis is placed upon investigations that are more directly linked to the treatment of clinical conditions.