Presence of Streptococcus infection in extra-oropharyngeal head and neck squamous cell carcinoma and its implication in carcinogenesis.
Presence of Streptococcus infection in extra-oropharyngeal head and neck squamous cell carcinoma and its implication in carcinogenesis.
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DOI：
10.3892/or.8.2.245
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发表时间：
2001-03
期刊：
影响因子：
4.2
通讯作者：
K. Shiga;M. Tateda;S. Saijo;Tetsuya Hori;Ikuro Sato;H. Tateno;K. Matsuura;T. Takasaka;T. Miyagi
中科院分区：
文献类型：
--
作者：
K. Shiga;M. Tateda;S. Saijo;Tetsuya Hori;Ikuro Sato;H. Tateno;K. Matsuura;T. Takasaka;T. Miyagi
调查S.为探讨咽峡炎菌在口咽外腔头颈部癌中的感染情况，我们对3例外耳道鳞癌和1例皮肤鳞癌的DNA标本进行了PCR和Southern杂交分析，以检测咽峡炎菌的DNA序列。心绞痛我们还检查了这四个标本的革兰氏染色，以检测链球菌的细菌体。通过PCR分析，获得了S. 4例（100%）肿瘤组织DNA中有4例（100%）存在心绞痛。Southern杂交结果显示，3例外耳道肿瘤中有1例（33%）阳性条带。我们用革兰氏染色法检测了外耳道癌肿瘤的三个标本中的一个和皮肤癌的一个标本中的链球菌菌体。与我们的预期相反，这些细菌体位于肿瘤的中间。自S.咽峡炎菌被认为是存在于口腔中的正常植物群，并且主要位于牙龈和牙菌斑中，这些数据强烈地表明S.心绞痛感染与头颈部鳞状细胞癌的发生有关。
To investigate the involvement of S. anginosus infection in head and neck cancer in the extra-oropharyngeal cavity, we analyzed 3 DNA samples prepared from squamous cell carcinoma of the external auditory canal and one from squamous cell carcinoma of the skin using polymerase chain reaction (PCR) analysis and Southern blot analysis to detect the DNA sequence of S. anginosus. We also examined these four specimens by Gram's stain to detect the streptococcal bacterial bodies. By PCR analysis, the DNA sequence of S. anginosus was found in 4 out of 4 (100%) DNA samples obtained from these tumors. By Southern blot analysis, positive bands were detected in one out of the 3 (33%) samples from the tumor taken from the external auditory canal. We detected streptococcal bacterial bodies in one of the three specimens from the tumor obtained from cancer of the external auditory canal and in the one specimen from the skin cancer by the method of Gram's stain. Contrary to our expectations, these bacterial bodies were located in the middle of the tumor. Since S. anginosus is thought to exist in the mouth as a normal flora and to be located mainly in the gingiva and dental plaque, these data strongly indicate that S. anginosus infection is implicated in the carcinogenesis of head and neck squamous cell carcinoma.