The geometric framework for nutrition reveals interactions between protein and carbohydrate during larval growth in honey bees.
The geometric framework for nutrition reveals interactions between protein and carbohydrate during larval growth in honey bees.
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DOI：
10.1242/bio.022582
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发表时间：
2017-06-15
期刊：
影响因子：
2.4
通讯作者：
Bowsher JH
中科院分区：
文献类型：
--
作者：
Helm BR;Slater GP;Rajamohan A;Yocum GD;Greenlee KJ;Bowsher JH
在全变态昆虫中，幼虫营养影响成虫的体型，这是一种对性能和健康有深远影响的生活史特征。个别幼虫的饮食营养成分往往是复杂的，可能会相互作用，有必要使用的几何框架来阐明营养效果。在蜜蜂中，蜜蜂保育蜂为发育中的幼虫提供食物，直接调节生长速度和等级发展。然而，蜜蜂的真社会性质使营养研究具有挑战性，因为在蜂巢中无法系统地操纵饮食成分。采用离体培养的方法，研究了碳水化合物和蛋白质含量对红腹锦鸡儿幼虫存活、生长和发育的影响。意利费拉。我们应用几何框架来确定这两种营养成分如何在九种人工饮食中相互作用。蜜蜂在广泛的蛋白质和碳水化合物含量下成功地完成了幼虫发育，其中中等蛋白质（1.5%）饮食具有最高的存活率。蛋白质和碳水化合物都有显着的和非线性的影响，生长速度最高的观察中蛋白质，低碳水化合物的饮食。饮食组成对发育时间没有统计学显著影响。这些结果证实了蛋白质和碳水化合物含量影响A.意大利蜜蜂幼虫然而，这项研究确定了碳水化合物和蛋白质含量之间的相互作用，表明低蛋白，高碳水化合物饮食对幼虫的生长和存活有负面影响。这些结果意味着，在低蛋白质条件下，即使花蜜丰富或蜂蜜储备充足，蜂巢中的工人招聘也会下降。总结：蛋白质与碳水化合物的比例影响体外工蜂幼虫的存活和生长速度。食物中的蛋白质和碳水化合物含量以复杂的方式相互作用，改变工蜂幼虫的生长速度、发育时间和存活率。
In holometabolous insects, larval nutrition affects adult body size, a life history trait with a profound influence on performance and fitness. Individual nutritional components of larval diets are often complex and may interact with one another, necessitating the use of a geometric framework for elucidating nutritional effects. In the honey bee, Apis mellifera, nurse bees provision food to developing larvae, directly moderating growth rates and caste development. However, the eusocial nature of honey bees makes nutritional studies challenging, because diet components cannot be systematically manipulated in the hive. Using in vitro rearing, we investigated the roles and interactions between carbohydrate and protein content on larval survival, growth, and development in A. mellifera. We applied a geometric framework to determine how these two nutritional components interact across nine artificial diets. Honey bees successfully completed larval development under a wide range of protein and carbohydrate contents, with the medium protein (∼5%) diet having the highest survival. Protein and carbohydrate both had significant and non-linear effects on growth rate, with the highest growth rates observed on a medium-protein, low-carbohydrate diet. Diet composition did not have a statistically significant effect on development time. These results confirm previous findings that protein and carbohydrate content affect the growth of A. mellifera larvae. However, this study identified an interaction between carbohydrate and protein content that indicates a low-protein, high-carb diet has a negative effect on larval growth and survival. These results imply that worker recruitment in the hive would decline under low protein conditions, even when nectar abundance or honey stores are sufficient. Summary: Protein to carbohydrate ratio affects the survival and growth rate of larval worker honey bees in vitro. Protein and carbohydrate content of food interact in complex ways to alter growth rate, development time and survival in worker bound honey bee larvae.