The single‐copy gene psbS codes for a phylogenetically intriguing 22 kDa polypeptide of photosystem II
The single‐copy gene psbS codes for a phylogenetically intriguing 22 kDa polypeptide of photosystem II
复制标题
DOI：
10.1016/0014-5793(92)81462-u
复制
发表时间：
1992-12
期刊：
影响因子：
3.5
通讯作者：
N. Wedell;R. Klein;U. Ljungberg;B. Andersson;R. G. Herrmann
中科院分区：
文献类型：
--
作者：
N. Wedell;R. Klein;U. Ljungberg;B. Andersson;R. G. Herrmann
从两个由聚腺苷化RNA或菠菜幼苗组成的λgt11表达文库中，用血清学方法选择了编码与光系统11相关的22 kDa多肽的完整前体多肽的重组噬菌体。这些cdna杂交成一个1.3 kb的RNA物种。前体蛋白由274个氨基酸残基组成，并携带约69个氨基酸残基的N端转运肽。成熟蛋白具有四个预测的跨膜片段，是光系统11的一个组成部分，起源于单拷贝基因。该蛋白的独特特征是：(1)它是具有两个膜跨度的祖先基因内部复制的结果。（ii）与LHC 1/11， CP24/CP29载脂蛋白和eip惊人的相似，尽管它不结合叶绿素并且存在于蓝藻中，并且，作为这些蛋白质，（iii）它整合到膜中，具有未裂解的路径信号，显示出与在二部转运多肽中发现的模式非常相似。
Recombinant phages that encode the complete precursor polypeptide for the 22 kDa polypeptide associated with photosystem 11 have been serologically selected from two λgt11 expression libraries made from polyadenylated RNA or spinach seedlings. The cDNAs hybridize to a 1.3 kb RNA species. The precursor protein is comprised of 274 amino acid residues and carries an N‐terminal transit peptide of probably 69 amino acid residues. The mature protein exhibits four, predicted transmembrane segments and is shown to be an integral component of photosystom 11 originating in a single‐copy gene. The unique characteristics of this protein are: (1) it is the result of a gene‐internal duplication of an ancestor with two membrane spans. (ii) a striking resemblance to LHC 1/11, CP24/CP29 apoproteins, and ELIPs, although it does not bind chlorophyll and is present in cyanobacteria, and, as these proteins, (iii) it integrates into the membrane with uncleaved routing signals that display remarkable resemblance to patterns found in bipartite transit pepticies.