Thailandins A and B, New Polyene Macrolactone Compounds Isolated from Actinokineospora bangkokensis Strain 44EHWT, Possessing Antifungal Activity against Anthracnose Fungi and Pathogenic Yeasts
Thailandins A and B, New Polyene Macrolactone Compounds Isolated from Actinokineospora bangkokensis Strain 44EHWT, Possessing Antifungal Activity against Anthracnose Fungi and Pathogenic Yeasts
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DOI：
10.1021/acs.jafc.6b01119
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发表时间：
2016-06-29
影响因子：
6.1
通讯作者：
Panbangred, Watanalai
中科院分区：
文献类型：
--
作者：
Intra, Bungonsiri;Greule, Anja;Panbangred, Watanalai
从曼谷放线菌44 EHW（T）的发酵液中分离得到两个新的多烯大环内酯类抗生素--泰国菌素A，1和B，2。采用半制备型HPLC和Sephadex LH-20凝胶过滤，同时进行抗真菌活性导向分级分离，从该菌株中分离纯化新化合物。通过UV、HR-ESI-MS和NMR等波谱技术鉴定了它们的结构。这些化合物对引起炭疽病的真菌具有广谱抗真菌活性（胶孢炭疽菌DoA d 0762、胶孢炭疽菌DoA c1060和辣椒炭疽菌DoA c1511）以及致病酵母（白色念珠菌MT 2013/1，寄生念珠菌DKMU 434，和新型隐球菌MT 2013/2），最小抑制浓度范围为16 - 32 μ g/mL。这是首次报道由放线菌产生的多烯抗生素作为生物活性化合物对炭疽真菌和致病酵母菌株。
Two new polyene macrolactone antibiotics, thailandins A, 1, and B, 2, were isolated from the fermentation broth of rhizosphere soil-associated Actinokineospora bangkokensis strain 44EHW(T). The new compounds from this strain were purified using semipreparative HPLC and Sephadex LH-20 gel filtration while following an antifungal activity guided fractionation. Their structures were elucidated through spectroscopic techniques including UV, HR-ESI-MS, and NMR. These compounds demonstrated broad spectrum antifungal activity against fungi causing anthracnose disease (Colletotrichum gloeosporioides DoA d0762, Colletotrichum gloeosporiodes DoA c1060, and Colletotrichum capsici DoA c1511) as well as pathogenic yeasts (Candida albicans MT 2013/1, Candida parasilopsis DKMU 434, and Cryptococcus neoformans MT 2013/2) with minimum inhibitory concentrations ranging between 16 and 32 mu g/mL. This is the first report of polyene antibiotics produced by Actinokineospora species as bioactive compounds against anthracnose fungi and pathogenic yeast strains.