CU,ZN SUPEROXIDE-DISMUTASE IS A PEROXISOMAL ENZYME IN HUMAN FIBROBLASTS AND HEPATOMA-CELLS
CU,ZN SUPEROXIDE-DISMUTASE IS A PEROXISOMAL ENZYME IN HUMAN FIBROBLASTS AND HEPATOMA-CELLS
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DOI：
10.1073/pnas.88.16.7381
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发表时间：
1991-08-01
影响因子：
11.1
通讯作者：
HALLEWELL, RA
中科院分区：
文献类型：
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作者：
KELLER, GA;WARNER, TG;HALLEWELL, RA
用4种单克隆抗Cu，Zn超氧化物歧化酶抗体，通过免疫荧光法检测了Cu，Zn超氧化物歧化酶（superoxide：superoxide oxidoreductase，EC 1.15.1.1）的细胞内定位。用过氧化物酶体基质酶过氧化氢酶共定位法研究了铜锌超氧化物歧化酶在人成纤维细胞和肝癌细胞中的过氧化物酶体定位。在齐薇格综合征患者的成纤维细胞中，该酶不被转运到过氧化物酶体鬼，但像过氧化氢酶一样，保留在细胞质中。此外，表达人Cu，Zn超氧化物歧化酶的酵母细胞的免疫冷冻电镜显示，该酶被易位到过氧化物酶体。
The intracellular localization of Cu,Zn superoxide dismutase (superoxide:superoxide oxidoreductase, EC 1.15.1.1) has been examined by immunofluorescence using four monoclonal anti-Cu,Zn superoxide dismutase antibodies raised against a recombinant human Cu,Zn superoxide dismutase derivative produced and purified from Escherichia coli. Colocalization with catalase, a peroxisomal matrix enzyme, was used to demonstrate the peroxisomal localization of Cu,Zn superoxide dismutase in human fibroblasts and hepatoma cells. In the fibroblasts of Zellweger syndrome patients, the enzyme is not transported to the peroxisomal ghosts but, like catalase, remains in the cytoplasm. In addition, immunocryoelectron microscopy of yeast cells expressing human Cu,Zn superoxide dismutase showed that the enzyme is translocated to the peroxisomes.