Simultaneous detection for three kinds of veterinary drugs: Chloramphenicol, clenbuterol and 17-beta-estradiol by high-throughput suspension array technology
Simultaneous detection for three kinds of veterinary drugs: Chloramphenicol, clenbuterol and 17-beta-estradiol by high-throughput suspension array technology
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高通量悬浮芯片技术同时检测氯霉素、瘦肉精、17-β-雌二醇三种兽药
DOI：
10.1016/j.aca.2008.10.061
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发表时间：
2009-01-19
影响因子：
6.2
通讯作者：
Chao, Fuhuan
中科院分区：
文献类型：
--
作者：
Liu, Nan;Su, Pu;Chao, Fuhuan
建立了同时检测氯霉素、克伦特罗和17-β-雌二醇三种兽药的悬浮芯片技术。合成并纯化了氯霉素和克伦特罗与牛血清白蛋白偶联物。将三种偶联物偶联在荧光微球/微珠上构成悬浮阵列探针，并通过扫描电镜观察微珠表面的微观结构，直接证实偶联物的偶联成功。分别对偶联物的最佳添加量和抗体的量进行了优化和选择。绘制标准曲线，决定系数R-2大于0.989，表明具有良好的逻辑相关性。三种兽药的检测范围分别为40-6.25 × 10（5）ng L-1、50-7.81 × 10（5）ng L-1和1 × 10（3）-7.29 × 10（5）ng L-1。最低检测限（LDLs）分别为40、50和1000 ngL（-1）。悬浮阵列具有特异性，与其他化学品无显著交叉反应性。同时将悬浮芯片法和ELISA法检测未知样品进行比较。两种方法对未知样品的检测结果与真实的结果误差较小，而悬浮芯片法的检测范围较传统ELISA法更广，灵敏度更高。高通量悬浮芯片技术操作简单、灵敏度高、成本低，是一种新型的兽药多组分分析方法。(C)2008 Elsevier B. V.保留所有权利。
Suspension array technology for simultaneous detection of three kinds of veterinary drugs, chloramphenicol (CAP), clenbuterol and 17-beta-estradiol has been developed. Conjugates of chloramphenicol and clenbuterol coupled with bovine serum albumin were synthesized and purified. Probes of suspension array were constituted by coupling the three conjugates on the fluorescent microspheres/beads and the microstructures of the beads' surface were observed by scanning electron microscopy which was a direct confirmation for the successful conjugates' coupling. The optimal addition of conjugates and the amounts of antibodies were optimized and selected, respectively. Standard curves were plotted and the coefficient of determination-R-2 was greater than 0.989 which suggested good logistic correlation. The detection ranges for the three veterinary drugs are 40-6.25 x 10(5) ng L-1, 50-7.81 x 10(5) ng L-1 and 1 x 10(3)-7.29 x 10(5) ngL(-1). respectively and the lowest detection limits (LDLs) of them are 40, 50 and 1000 ngL(-1), respectively. The suspension array is specific and has no significant cross-reactivity with other chemicals. Meanwhile, unknown samples were detected by suspension array and ELISA in comparison with each other. The errors between found and real for the detection of the unknown samples were relatively small to both of the two methods, whereas, the detection ranges of suspension array are broader and sensitive than that of the traditional ELISA. The high-throughput suspension array is proved to be a novel method for multi-analysis of veterinary drugs with simple operation, high sensitivity and low cost. (C) 2008 Elsevier B.V. All rights reserved.