DOES TRANS-LESION SYNTHESIS EXPLAIN THE UV-RADIATION RESISTANCE OF DNA-SYNTHESIS IN C-ELEGANS EMBRYOS
DOES TRANS-LESION SYNTHESIS EXPLAIN THE UV-RADIATION RESISTANCE OF DNA-SYNTHESIS IN C-ELEGANS EMBRYOS
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DOI：
10.1016/0921-8777(91)90050-y
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发表时间：
1991-09-01
期刊：
影响因子：
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通讯作者：
SVENDSEN, BA
中科院分区：
文献类型：
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作者：
HARTMAN, P;REDDY, J;SVENDSEN, BA
与其他模型系统或线虫 rad 突变体相比，抑制野生型线虫胚胎中的 DNA 合成和细胞分裂需要超过 10 倍的通量。此外，与其他生物体不同，子DNA链的分子量只有在大的、超致死的通量之后才会降低。新生DNA片段的分子量超过二聚体间距离高达19倍，表明线虫胚胎可以通过非指导性损伤进行复制。这种假定的跨损伤合成能力可以解释紫外线辐射对秀丽隐杆线虫胚胎 DNA 合成和核分裂的难熔性质。
Over 10-fold larger fluences were required to inhibit both DNA synthesis and cell division in wild-type C elegans embryos as compared with other model systems or C elegans rad mutants. In addition, unlike in other organisms, the molecular weight of daughter DNA strands was reduced only after large, superlethal fluences. The molecular weight of nascent DNA fragments exceeded the interdimer distance by up to 19-fold, indicating that C elegans embryos can replicate through non-instructional lesions. This putative trans-lesion synthetic capability may explain the refractory nature of UV radiation on embryonic DNA synthesis and nuclear division in C elegans.