Total Flavonoids from Gnaphalium affine D. Don Exert Analgesic Effect via Inhibiting the Production of Pro-inflammatory Mediators in Mouse Model of Pain
Total Flavonoids from Gnaphalium affine D. Don Exert Analgesic Effect via Inhibiting the Production of Pro-inflammatory Mediators in Mouse Model of Pain
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发表时间：
2014
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通讯作者：
Huang Xiao-ji
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作者：
Huang Xiao-ji
目的：探讨仿射钠总黄酮的抗伤害作用及其机制。方法：采用热致疼痛小鼠模型和醋酸腹腔注射模型，观察口服总黄酮的镇痛作用。同时测定小鼠血浆中肿瘤坏死因子-α(TNF-α)、白细胞介素-6（IL-6）、一氧化氮（NO）等细胞因子的产生。同时检测腹腔巨噬细胞中TNF-α、IL-6和诱导型一氧化氮合酶（inos）的基因表达水平。结果：总黄酮显著增加小鼠热板戒断潜伏期，抑制小鼠扭体反应。此外，总黄酮可抑制血浆中TNF-α、IL-6、NO的生成，降低腹腔巨噬细胞中TNF-α、IL-6、i - NOS的表达水平。结论：仿射草总黄酮对小鼠具有镇痛作用，其作用机制可能与抑制血浆细胞因子的产生和巨噬细胞介导的炎症反应有关。
Objective: To investigate the anti-nociceptive effect of total flavonoids from Gnaphalium affine D. Don and the involved mechanism. Methods: Employing the mouse model of pain induced by heat and intraperitoneal injection of acetic acid, the analgesic activity of oral administration of the total flavonoids was assessed. Moreover, the production of cytokines in mouse plasma, including tumor necrosis factor-α(TNF-α), interleukin-6(IL-6) and nitric oxide(NO) was determined. The gene expression levels of TNF-α, IL-6 and inducible nitric oxide synthase(i NOS) in peritoneal macrophages were also examined. Results: The total flavonoids significantly increased the withdrawal latency in hot plate tests and inhibited the writhing response in mice. In addition, the total flavonoids blocked the production of TNF-α, IL-6, and NO in plasma and reduced the expression levels of TNF-α, IL-6, and i NOS in the peritoneal macrophages. Conclusions: The total flavonoids from Gnaphalium affine D. Don exert analgesic effects in mice, which is associated with the inhibition of the production of cytokines in plasma and macrophage-mediated inflammation.