Redox ratio of mitochondria as an indicator for the response of photodynamic therapy.
Redox ratio of mitochondria as an indicator for the response of photodynamic therapy.
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DOI：
10.1117/1.1760759
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发表时间：
2004-07
影响因子：
3.5
通讯作者：
Zhihong Zhang;Dana Blessington;Hui Li;T. Busch;J. Glickson;Qingming Luo;B. Chance;G. Zheng
中科院分区：
文献类型：
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作者：
Zhihong Zhang;Dana Blessington;Hui Li;T. Busch;J. Glickson;Qingming Luo;B. Chance;G. Zheng
通过肿瘤氧化还原状态的成像研究光动力疗法(PDT)对肿瘤线粒体代谢状态的影响。PDT是使用光敏剂焦脱镁叶绿酸-2-脱氧葡糖胺(PYRO-2DG)进行的，它利用葡萄糖输入途径。研究发现，Pyro-2DG诱导的PDT导致肿瘤线粒体处于高度氧化状态。这是由使用冷冻成像仪观察到的内在氧化黄素蛋白(FP)和还原的吡啶核苷酸(PN)[即还原的烟酰胺腺嘌呤二核苷酸(NADH)]荧光信号产生的氧化还原比变化确定的。因此，氧化还原比率是一个灵敏的指标，可以为PDT诱导的组织损伤提供可靠和信息的测量。在肿瘤的PDT治疗区域，检测到高度氧化的黄素蛋白和减弱的NADH荧光，表明黄素蛋白和NADH被光敏化过程中产生的单线态氧氧化。
The effect of photodynamic therapy (PDT) treatment on the metabolic state of tumor mitochondria is investigated by imaging of tumor redox status. PDT is performed using the photosensitizer pyropheophorbide-2-deoxyglucosamide (Pyro-2DG), which utilizes the glucose import pathway. It is found that Pyro-2DG-induced PDT resulting in a highly oxidized state of tumor mitochondria. This is determined from the redox ratio changes derived from the intrinsic oxidized flavoprotein (Fp) and reduced pyridine nucleotide (PN) [i.e., reduced nicotinamide adenine dinucleotide (NADH)] fluorescence signals observed using a cryoimager. Thus, the redox ratio is a sensitive indicator for providing reliable and informative measurements of PDT-induced tissue damage. In the PDT treated region of the tumor, highly oxidized flavoprotein and diminishing NADH fluorescence is detected, suggesting that flavoprotein and NADH are oxidized by singlet oxygen produced in the photosensitization process.