Laser light is capable of inducing proliferation of carcinoma cells in culture:: A spectroscopic in vitro study
Laser light is capable of inducing proliferation of carcinoma cells in culture:: A spectroscopic in vitro study
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DOI：
10.1089/pho.2005.23.300
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发表时间：
2005-06-01
影响因子：
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通讯作者：
De Castro, JLF
中科院分区：
文献类型：
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作者：
Werneck, CE;Pinheiro, ALB;De Castro, JLF
目的：本工作的目的是通过MTT法评估细胞活力，评估时间、处理和波长对H.Ep.2细胞增殖的影响，所述H.Ep.2细胞接受或不接受具有相同能量密度（4 J/cm（2））的激光照射（λ 685和λ 830 nm）。背景资料：虽然恶性病变的研究已经有一段时间了，但还没有确定的治愈方法。如果在发育的初始阶段诊断出病变，死亡率可以降低。材料和方法：H.Ep.2细胞在烧瓶中培养，并在DMEN培养基（10%FBS，1%L-谷氨酰胺和1%抗生素溶液）中维持。对于照射，将细胞保存在含有DMEM培养基（5%FBS、1%L-谷氨酰胺和1%抗生素溶液）的96孔板的24个威尔斯孔中，用波长为λ 685-和λ 830-nm的激光照射，并在照射后0、6、12、24和48小时染色。结果：三组比较有显著性差异（p = 0.0087）。λ 685 nm（p = 0.0202）和λ 830 nm（p = 0.0324）照射组之间存在显著差异。照射时间仅显著影响λ 685-nm组（p = 0.04）。波长具有显著影响（p = 0.013）。结论：时间、处理和波长显著影响H.Ep.2细胞的增殖过程。
Objective: The objective of this work was to evaluate the influence of time, treatment, and wavelength, through the assessment of the cellular viability with MTT, on the proliferation of H.Ep.2 cells subjected to laser irradiation or not (lambda 685 and lambda 830 nm) with the same energy density (4 J/cm(2)). Background Data: Although malignant lesions have been studied for some time, there is not yet a definitive cure. Mortality could be reduced if lesions were diagnosed on initial phases of development. Materials and Methods: H.Ep.2 cells were cultured in flasks and maintained in DMEN medium (10% FBS, 1% L-glutamine, and 1% antibiotic solution). For irradiation, cells were kept in 24 wells of the 96-well plaques containing DMEM medium (5% FBS, 1% L-glutamine, and 1% antibiotic solution), irradiated with lasers at lambda 685- and lambda 830-nm wavelength, and stained at 0, 6, 12, 24, and 48 h after irradiation. Results: There was significant differences when the three groups were compared (p = 0.0087). There was significant difference for both irradiated groups, lambda 685 nm (p = 0.0202) and lambda 830 nm (p = 0.0324). Time of irradiation significantly influenced only the lambda 685-nm group (p = 0.04). The wavelength had a significant influence (p = 0.013). Conclusion: Time, treatment, and wavelength significantly influenced the proliferation process of H.Ep.2 cells.