A TP-FRET-based two-photon fluorescent probe for ratiometric visualization of endogenous sulfur dioxide derivatives in mitochondria of living cells and tissues
A TP-FRET-based two-photon fluorescent probe for ratiometric visualization of endogenous sulfur dioxide derivatives in mitochondria of living cells and tissues
复制标题
基于 TP-FRET 的双光子荧光探针，用于活细胞和组织线粒体中内源二氧化硫衍生物的比率可视化
DOI：
10.1039/c6cc05254a
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发表时间：
2016
影响因子：
4.9
通讯作者：
Yang Ronghua
中科院分区：
文献类型：
--
作者：
Yang Xiaoguang;Zhou Yibo;Zhang Xiufang;Yang Sheng;Chen Yun;Guo Jingru;Li Xiaoxuan;Qing Zhihe;Yang Ronghua
首次提出了一种基于丙酮-巯基二联体的SO2衍生物比率型双光子荧光探针。它被成功地应用于可视化的酶促产生的SO2衍生物的波动，在线粒体的HepG 2细胞和大鼠肝组织，使用双光子荧光显微镜成像。
A ratiometric two-photon fluorescent probe for SO2 derivatives was first proposed based on acedan–merocyanine dyads via a TP-FRET strategy. It was successfully applied to visualization of the fluctuations of enzymatically generated SO2 derivatives in the mitochondria of HepG2 cells and rat liver tissues using two-photon fluorescence microscopy imaging.