Three-dimensional reconstruction with contrast transfer function correction from energy-filtered cryoelectron micrographs: Procedure and application to the 70S Escherichia coli ribosome
Three-dimensional reconstruction with contrast transfer function correction from energy-filtered cryoelectron micrographs: Procedure and application to the 70S Escherichia coli ribosome
复制标题
DOI：
10.1006/jsbi.1997.3845
复制
发表时间：
1997-04-01
影响因子：
3
通讯作者：
Frank, J
中科院分区：
文献类型：
--
作者：
Zhu, J;Penczek, PA;Frank, J
冷冻电子显微镜提供了研究天然状态大分子的方法。然而，对比度传递函数 (CTF) 使得图像和由它们导出的三维 (3D) 地图难以解释。我们开发了根据实验数据确定 CTF 并获得 CTF 校正的 3D 重建的方法。一步完成的CTF校正和3D重建，可以轻松组合不同的离焦数据集，减少计算中的误差累积。这些方法应用于 70S 大肠杆菌核糖体的能量过滤图像，产生分辨率为 1/24.5 埃 (-1) 的无失真核糖体 3D 图，由微分相位残差分辨率标准确定。 (C) 1997 年学术出版社。
Cryoelectron microscopy provides the means of studying macromolecules in their native state. However, the contrast transfer function (CTF) makes the images and the three-dimensional (3D) maps derived from them difficult to interpret. We developed methods to determine the CTF from experimental data and to obtain a CTF-corrected 3D reconstruction. The CTF correction and 3D reconstruction accomplished in one step make it easy to combine different defocus data sets and decrease the error accumulation in the computation. These methods were applied to energy-filtered images of the 70S Escherichia coli ribosome, resulting in a distortion-free 3D map of the ribosome at 1/24.5 Angstrom(-1) resolution, as determined by the differential phase residual resolution criterion. (C) 1997 Academic Press.