Microbioassay system for an anti-cancer agent test using animal cells on a microfluidic gradient mixer
Microbioassay system for an anti-cancer agent test using animal cells on a microfluidic gradient mixer
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DOI：
10.2116/analsci.22.87
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发表时间：
2006-01-01
影响因子：
1.6
通讯作者：
Sato, K
中科院分区：
文献类型：
--
作者：
Fujii, S;Uematsu, M;Sato, K
我们开发了一种新型的微生物测定系统，配备了两种溶液的梯度混合器，我们将微流控系统应用于使用活动物细胞在微芯片上的抗癌剂测试。采用软光刻法在聚二甲基硅氧烷基底上制作了一个用于两种溶液梯度混合的微通道和八个用于细胞分析的微通道。该生物测定所需的功能，即，将细胞培养、化学刺激、细胞染色和荧光测定集成到微流控芯片中。在微芯片上以0.1 μ l/min的速度保存8个梯度浓度的荧光素溶液，范围从1 μ g/ml到98 μ g/ml。培养胃癌细胞系，并在微芯片上使用5-氟尿嘧啶作为抗癌剂进行细胞活力测定。细胞活力根据试剂溶液的估计浓度而变化。使用微生物测定系统，使用活细胞同时在8个单独的通道中进行抗癌剂测试，其中试剂的浓度梯度在微芯片上。
We developed a novel microbioassay system equipped with a gradient mixer of two solutions, and we applied the microfluidic system to an anti-cancer agent test using living animal cells on a microchip. A microchannel for the gradient mixing of two solutions and eight other microchannels for cell assay were fabricated on a poly(dimethylsiloxane) substrate using a soft-lithography method. The functions necessary for this bioassay, i.e., cell culturing, chemical stimulation, cell staining, and fluorescence determination, were integrated into the microfluidic chip. Eight gradient concentrations of the fluorescein solution, ranging from 1 to 98 mu g/ml, were archived at 0.1 mu l/min on a microchip. A stomach cancer cell line was cultured, and a cell viability assay was conducted using 5-Fluorouracil as an anti-cancer agent on the microchip. Cell viability changed according to the estimated concentration of the agent solution. With the microbioassay system, an anti-cancer agent test was conducted using living cells simultaneously in eight individual channels with the gradient concentration of the agent on a microchip.