The immunolocalization of protein gene product 9.5 using rabbit polyclonal and mouse monoclonal antibodies.
The immunolocalization of protein gene product 9.5 using rabbit polyclonal and mouse monoclonal antibodies.
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发表时间：
1988-02
影响因子：
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通讯作者：
P. Wilson;Pc Barber;B. Power;Q. Hamid;A. Dhillon;J. Rode;Inm Day;R. Thompson;J. Polak
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作者：
P. Wilson;Pc Barber;B. Power;Q. Hamid;A. Dhillon;J. Rode;Inm Day;R. Thompson;J. Polak
为了评估蛋白基因产物（PGP）9.5作为神经和神经内分泌系统的标志物的潜力，我们研究了其在人类，大鼠和豚鼠组织中的免疫定位，使用兔多克隆抗血清和两种新的小鼠单克隆抗血清，I3C4和3IA3。我们的研究结果表明，免疫反应阳性的PGP 9.5在神经元和神经纤维在所有级别的中枢和外周神经系统，在许多神经内分泌细胞，在部分肾小管，精原细胞和睾丸间质细胞，并在卵子和一些细胞的怀孕和非怀孕的黄体。在常规处理的组织中，使用多克隆抗体的标准免疫组织化学技术显示了各种大小的外周神经纤维，具有惊人的清晰度。
In order to assess the potential of protein gene product (PGP) 9.5 as a marker of the nervous and neuroendocrine systems, we examined its immunolocation in human, rat and guinea-pig tissues, using a rabbit polyclonal antiserum and two new mouse monoclonal antisera, I3C4 and 3IA3. Our results demonstrate immunoreactive PGP 9.5 in neurons and nerve fibres at all levels of the central and peripheral nervous system, in many neuroendocrine cells, in part of the renal tubule, in spermatogonia and Leydig cells of the testis, and in ova and in some cells of the pregnant and non-pregnant corpus luteum. In routinely processed tissues, standard immunohistochemical techniques using the polyclonal antibody demonstrated peripheral nerve fibres of all sizes with striking clarity.