A Novel Small Molecular Antibody, HER2-Nanobody, Inhibits Tumor Proliferation in HER2-Positive Breast Cancer Cells In Vitro and In Vivo.
A Novel Small Molecular Antibody, HER2-Nanobody, Inhibits Tumor Proliferation in HER2-Positive Breast Cancer Cells In Vitro and In Vivo.
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一种新型的小分子抗体HER2-纳米病，在体外和体内抑制HER2阳性乳腺癌细胞中的肿瘤增殖。
DOI：
10.3389/fonc.2021.669393
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发表时间：
2021
影响因子：
4.7
通讯作者：
Ding K
中科院分区：
文献类型：
--
作者：
Yan Y;Cheng X;Li L;Zhang R;Zhu Y;Wu Z;Ding K
乳腺癌是世界范围内，特别是发展中国家妇女最常见的恶性肿瘤。赫赛汀是一种单克隆抗体，对HER 2阳性乳腺癌具有抗肿瘤作用。然而，赫赛汀的大分子量限制了其应用。在本研究中，我们构建并筛选了HER 2-纳米抗体，并验证了其在HER 2阳性乳腺癌细胞中的肿瘤抑制作用。构建、筛选、纯化HER 2-纳米抗体，并在体外证明其抑制HER 2阳性乳腺癌细胞的细胞总数、活力、集落形成和有丝分裂，并促进细胞凋亡。用HER 2-纳米抗体处理，肿瘤生长通过体内瘤内注射和尾静脉内注射均被显著抑制。HER 2-nanobody抑制HER 2阳性乳腺癌细胞中ERK和AKT的磷酸化。RAS-RAF-MAPK和PI 3 K-AKT-mTOR是HER 2的两条重要通路。HER 2-nanobody可能通过抑制ERK和AKT的磷酸化而发挥抑瘤作用。因此，HER 2-纳米抗体可用作抑制HER 2阳性乳腺癌的小分子抗体。
Breast cancer is the most common malignant cancer in women worldwide, especially in developing countries. Herceptin is a monoclonal antibody with an antitumor effect in HER2-positive breast cancer. However, the large molecular weight of Herceptin limited its employment. In this study, we constructed and screened HER2-nanobody and verified its tumor-suppressive effect in HER2-positive breast cancer cells. HER2-nanobody was established, filtrated, purified, and was demonstrated to inhibit cell total number, viability, colony formation and mitosis, and promote cell apoptosis in HER2-positive breast cancer cells in vitro. Treated with HER2-nanobody, tumor growth was significantly inhibited by both intratumor injection and tail intravenous injection in vivo. The phosphorylation of ERK and AKT was restrained by HER2-nanobody in HER2-positive breast cancer cells. RAS-RAF-MAPK and PI3K-AKT-mTOR are two important pathways involved in HER2. It was credible for HER2-nanobody to play the tumor suppressive role by inhibiting the phosphorylation of ERK and AKT. Therefore, HER2-nanobody could be employed as a small molecular antibody to suppress HER2-positive breast cancer.
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影响因子：
11.1
作者：
Liu, Qi;Borcherding, Nicholas C.;Qi, Hank H.
通讯作者：
Qi, Hank H.
影响因子：
3.6
作者：
Bradley, M. E.;Dombrecht, B.;Cromie, K. D.
通讯作者：
Cromie, K. D.
影响因子：
4.8
作者：
Deb, TB;Su, L;Johnson, GR
通讯作者：
Johnson, GR
DOI：
10.1007/978-1-61779-968-6_18
发表时间：
2012-01-01
期刊：
Methods in molecular biology (Clifton, N.J.)
影响因子：
--
作者：
Agrawal, Vishal;Slivac, Igor;Durocher, Yves
通讯作者：
Durocher, Yves
影响因子：
4.8
作者：
Maussang, David;Mujic-Delic, Azra;Smit, Martine J.
通讯作者：
Smit, Martine J.