Herpetol ameliorates allergic contact dermatitis through regulating T-lymphocytes
Herpetol ameliorates allergic contact dermatitis through regulating T-lymphocytes
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Herpetol 通过调节 T 淋巴细胞改善过敏性接触性皮炎
DOI：
10.1016/j.intimp.2016.08.025
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发表时间：
2016
影响因子：
5.6
通讯作者：
Xu Qiang
中科院分区：
文献类型：
--
作者：
Li Xiang;Wang Xingqi;Jiang Hezhong;Zhang Guohui;Tan Rui;Sun Yang;Wu Xuefeng;Tan Renxiang;Xu Qiang
越来越多的过敏性接触性皮炎患者仍然迫切需要一种副作用较小的免疫抑制剂。在本研究中，我们旨在通过体外和体内实验研究蛇足醇对t淋巴细胞的免疫抑制活性，并探讨其潜在的药理机制。结果表明，在5 ~ 20 μM的刺激下，海鞘醇能有效抑制活化T细胞的增殖，减少促炎细胞因子的产生。此外，20 ~ 40 mg/kg的海参醇可显著改善苦味氯诱导的耳部肿胀和炎症反应。此外，herpetol可能以剂量依赖的方式引起活化T细胞的细胞周期阻滞。此外，海鞘醇降低HIF-1α、Glut1和LDHA的表达和活性，导致活化T细胞糖酵解抑制。综上所述，在体内和体外实验中，herpetol对t细胞介导的免疫反应具有免疫抑制活性，具有治疗免疫相关皮肤病的潜力。
An immunosuppressant with fewer adverse effects is still urgently needed for increasing numbers of patients suffering from allergic contact dermatitis. In the present study, we aimed to investigate the immunosuppressive activity of herpetol on T-lymphocytes in vitro and in vivo and explore its potential pharmacological mechanism. The results showed that herpetol could effectively inhibit the proliferation of activated T cells and reduce the production of pro-inflammatory cytokines at 5–20 μM. Additionally, the ear swelling and inflammatory responses induced by picryl chloride were significantly ameliorated by herpetol at 20–40 mg/kg. Moreover, herpetol could cause cell cycle arrest of activated T cells in a dose-dependent manner. Furthermore, herpetol reduced the expression and activity of HIF-1α, Glut1 and LDHA, leading to glycolysis inhibition in activated T cells. Taken together, herpetol showed an immunosuppressive activity against T-cell mediated immune responses in vitro and in vivo, and it has potential for the treatment of immune-related skin diseases.