Enhancing Photostability of a Coumarin Dye by Self‐inclusion into a Cyclodextrin Cavity in Aqueous Solution and Living Cells
Enhancing Photostability of a Coumarin Dye by Self‐inclusion into a Cyclodextrin Cavity in Aqueous Solution and Living Cells
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DOI：
10.1002/ajoc.202000365
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发表时间：
2020-10
影响因子：
2.7
通讯作者：
Hajime Shigemitsu;Keigo Matsuda;Tadashi Mori;H. Nakatsuji;M. Matsusaki;T. Kida
中科院分区：
文献类型：
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作者：
Hajime Shigemitsu;Keigo Matsuda;Tadashi Mori;H. Nakatsuji;M. Matsusaki;T. Kida
光稳定性有机染料对于长时间或超分辨率生物成像具有很高的需求。在此，我们证明了有效的改善香豆素染料（太平洋蓝（PB））的光稳定性的空间位阻保护使用环糊精。PB共轭环糊精（PB−CD）的体外光稳定性比PB乙基酰胺（PB−EA）高2.8倍，PB乙基酰胺是一种比较化合物。 环糊精与PB的结合不仅可以保护PB单元免受化学反应的影响，而且可以抑制导致染料降解的活性氧（ROS）的产生。这些效应使PB染料的光稳定性显著提高。最后，使用共聚焦激光扫描显微镜（CLSM）评估PB − CD的光稳定性，即使在活细胞中也证明了显着的改善效果。
Photostable organic dyes are highly demanded for longtime or super‐resolution bioimaging. Herein, we demonstrate effective improvement of photostability of a coumarin dye (Pacific Blue (PB)) by steric protection using a cyclodextrin. ThePBconjugated cyclodextrin (PB−CD) showed 2.8 times higher photostability than PB ethyl amide (PB−EA) which is a comparative compound in vitro. The cyclodextrin conjugation toPBnot only protectPBunit from reactive chemicals, but also suppress reactive oxygen species (ROS) generation which causes dye degradation. The effects afford significant improvement of photostability ofPBdye. Finally, the photostability ofPB−CDwas evaluated using confocal laser scanning microscopy (CLSM) and the significant improvement effect was proofed even in living cells.