Acylation monitoring in solid phase peptide synthesis by the equilibrium distribution of coloured ions
Acylation monitoring in solid phase peptide synthesis by the equilibrium distribution of coloured ions
复制标题
通过有色离子的平衡分布监测固相肽合成中的酰化
DOI：
10.1039/c39900000538
复制
发表时间：
1990
期刊：
影响因子：
--
通讯作者：
D. Owen
中科院分区：
文献类型：
--
作者：
S. Salisbury;E. J. Tremeer;J. Davies;D. Owen
分光光度法监测固相肽合成中酰化步骤期间阴离子染料从载体基质中的释放（清洁发展机制）是这一过程中经常出现问题的部分的进展的敏感指标;该新方法容易结合到自动化仪器中，其中它允许对偶联反应所允许的时间进行反馈控制，从而提高了产品质量和合成速度。
Spectrophotometric monitoring of the release of anionic dye from the support matrix during the acylation step in solid phase peptide synthesis (counterion distribution monitoring; CDM) is a sensitive indicator of the progress of this often problematic part of the process; this new method is readily incorporated into automated instrumentation where it permits feedback control of the time allowed for the coupling reaction, with consequent improvements in product quality and the speed of synthesis.