Assembly and function of the archaeal flagellum
Assembly and function of the archaeal flagellum
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DOI：
10.1042/bst0390064
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发表时间：
2011-02-01
影响因子：
3.9
通讯作者：
Albers, Sonja-Verena
中科院分区：
文献类型：
--
作者：
Ghosh, Abhrajyoti;Albers, Sonja-Verena
运动是原核生物的一种常见行为。细菌和古细菌都利用鞭毛来游动，但有充分的证据表明，这两种生命领域的鞭毛结构完全不同，尽管它们的功能相似。有趣的是，迄今为止已有的信息表明，在结构上，古细菌鞭毛更类似于细菌IV型毛，而不是细菌鞭毛。随着古细菌基因组序列信息的不断增加和遗传工具的进步，鉴定古细菌鞭毛组装过程中涉及的成分成为可能。这些成分的一个子集与IV型菌枝组装系统的成分相似。尽管参与古菌鞭毛组装的分子分子正在被确定，但古菌鞭毛组装的力学和动力学尚未建立。我们实验室最近的计算分析已经在鞭毛的核心蛋白之一，膜整合蛋白flj中发现了保守的高电荷环区域，并预测这些区域与组装atp酶FlaI的相互作用有关。有趣的是，在古细菌的两个主要亚界——Euryarchaeota和Crenarchaeota——中发现了相当大的差异。了解组装途径并创建古细菌鞭毛分子参与者的相互作用图将揭示组装的细节以及与细菌IV型毛组装系统的进化关系。
Motility is a common behaviour in prokaryotes. Both bacteria and archaea use flagella for swimming motility, but it has been well documented that structures of the flagellum from these two domains of life are completely different, although they contribute to a similar function. Interestingly, information available to date has revealed that structurally archaeal flagella are more similar to bacterial type IV pili rather than to bacterial flagella. With the increasing genome sequence information and advancement in genetic tools for archaea, identification of the components involved in the assembly of the archaeal flagellum is possible. A subset of these components shows similarities to components from type IV pilus-assembly systems. Whereas the molecular players involved in assembly of the archaeal flagellum are being identified, the mechanics and dynamics of the assembly of the archaeal flagellum have yet to be established. Recent computational analysis in our laboratory has identified conserved highly charged loop regions within one of the core proteins of the flagellum, the membrane integral protein FlaJ, and predicted that these are involved in the interaction with the assembly ATPase FlaI. Interestingly, considerable variation was found among the loops of FlaJ from the two major subkingdoms of archaea, the Euryarchaeota and the Crenarchaeota. Understanding the assembly pathway and creating an interaction map of the molecular players in the archaeal flagellum will shed light on the details of the assembly and also the evolutionary relationship to the bacterial type IV pili-assembly systems.