Animal microRNAs confer robustness to gene expression and have a significant impact on 3′UTR evolution
Animal microRNAs confer robustness to gene expression and have a significant impact on 3′UTR evolution
复制标题
DOI：
10.1016/j.cell.2005.11.023
复制
发表时间：
2005-12-16
期刊：
影响因子：
64.5
通讯作者：
Cohen, SM
中科院分区：
文献类型：
--
作者：
Stark, A;Brennecke, J;Cohen, SM
microRNA是高等真核生物中作为基因表达的转录后调节因子的小的非编码RNA。最近估计，所有基因中有20%-30%是微小RNA靶点，这凸显了它们在动物中的广泛而重要的作用。在这里，我们报告说，大量的基因参与基本的细胞过程，避免microRNA的调节，由于短的3 'UTR是专门耗尽的microRNA结合位点。对于单个microRNA，我们发现共表达基因避开microRNA位点，而靶基因和microRNA优先在邻近组织中表达。这种相互排斥的表达表明，microRNA赋予发育基因表达程序以准确性，从而确保组织身份并支持细胞谱系决策。
MicroRNAs are small noncoding RNAs that serve as posttranscriptional regulators of gene expression in higher eukaryotes. Their widespread and important role in animals is highlighted by recent estimates that 20%-30% of all genes are microRNA targets. Here, we report that a large set of genes involved in basic cellular processes avoid microRNA regulation due to short 3'UTRs that are specifically depleted of microRNA binding sites. For individual microRNAs, we find that coexpressed genes avoid microRNA sites, whereas target genes and microRNAs are preferentially expressed in neighboring tissues. This mutually exclusive expression argues that microRNAs confer accuracy to developmental gene-expression programs, thus ensuring tissue identity and supporting cell-lineage decisions.