Gene expression in ovarian cancer reflects both morphology and biological behavior, distinguishing clear cell from other poor-prognosis ovarian carcinomas.
Gene expression in ovarian cancer reflects both morphology and biological behavior, distinguishing clear cell from other poor-prognosis ovarian carcinomas.
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DOI：
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发表时间：
2002-08
期刊：
影响因子：
11.2
通讯作者：
D. Schwartz;S. Kardia;K. Shedden;R. Kuick;G. Michailidis;Jeremy M. G. Taylor;David E. Misek;R. Wu
中科院分区：
文献类型：
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作者：
D. Schwartz;S. Kardia;K. Shedden;R. Kuick;G. Michailidis;Jeremy M. G. Taylor;David E. Misek;R. Wu
长期以来，人们一直在寻找具有生物学和临床意义的肿瘤分类方案。一些恶性上皮肿瘤，如甲状腺和子宫内膜的肿瘤，表现出不止一种分化模式，每种分化模式都与独特的临床特征和治疗方法相关。在其他组织中，所有的癌，无论形态类型如何，都被当作一种疾病来治疗。为了更好地了解卵巢癌（OvCa）四种主要组织学类型的生物学和临床特征，我们使用寡核苷酸芯片分析了113例卵巢上皮肿瘤的基因表达。使用PCA获得基因表达变化的全局视图。这些分析表明，无论肿瘤分期和分级如何，基于其基因表达谱，粘液细胞和透明细胞OvCas可以很容易地与浆液细胞OvCas区分开来。相反，子宫内膜样腺癌与其他组织学类型有明显的重叠。虽然高分期/分级肿瘤通常可与低分期/分级肿瘤区分开来，但透明细胞OvCa具有将其与其他不良预后OvCa区分开来的分子特征。事实上，有73个基因在透明细胞OvCa中的表达比其他OvCa类型高2到29倍。总的来说，这些数据表明卵巢腺癌的基因表达模式反映了形态学特征和生物学行为。此外，这些研究为卵巢癌新的类型特异性诊断策略和治疗的发展提供了基础。
Biologically and clinically meaningful tumor classification schemes have long been sought. Some malignant epithelial neoplasms, such as those in the thyroid and endometrium, exhibit more than one pattern of differentiation, each associated with distinctive clinical features and treatments. In other tissues, all carcinomas, regardless of morphological type, are treated as though they represent a single disease. To better understand the biological and clinical features seen in the four major histological types of ovarian carcinoma (OvCa), we analyzed gene expression in 113 ovarian epithelial tumors using oligonucleotide microarrays. Global views of the variation in gene expression were obtained using PCA. These analyses show that mucinous and clear cell OvCas can be readily distinguished from serous OvCas based on their gene expression profiles, regardless of tumor stage and grade. In contrast, endometrioid adenocarcinomas show significant overlap with other histological types. Although high-stage/grade tumors are generally separable from low-stage/grade tumors, clear cell OvCa has a molecular signature that distinguishes it from other poor-prognosis OvCas. Indeed, 73 genes, expressed 2- to 29-fold higher in clear cell OvCas compared with each of the other OvCa types, were identified. Collectively, the data indicate that gene expression patterns in ovarian adenocarcinomas reflect both morphological features and biological behavior. Moreover, these studies provide a foundation for the development of new type-specific diagnostic strategies and treatments for ovarian cancer.