SEQUENCE OF THE SPIKE PROTEIN OF THE PORCINE EPIDEMIC DIARRHEA VIRUS
SEQUENCE OF THE SPIKE PROTEIN OF THE PORCINE EPIDEMIC DIARRHEA VIRUS
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DOI：
10.1099/0022-1317-75-5-1195
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发表时间：
1994-05-01
影响因子：
3.8
通讯作者：
LAUDE, H
中科院分区：
文献类型：
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作者：
DUARTE, M;LAUDE, H
从cDNA克隆中测定了猪流行性腹泻病毒（PEDV） Br1/87分离株穗(S)基因的完整序列。预测的多肽长1383个氨基酸，含有29个潜在的n -链糖基化位点，其结构特征与冠状病毒刺突蛋白相似。与传染性胃肠炎病毒（TGEV）相关亚群成员一样，PEDV S蛋白缺乏蛋白水解位点以产生裂解的氨基和羧基S1和S2。在pedv感染的细胞中观察到预期M(r)的病毒多肽种类，即170K/190K。序列比较证实，在该亚群中，PEDV与人呼吸道冠状病毒HCV 229E关系最为密切。然而，PEDV S蛋白有额外的250个残基n端结构域，这在HCV 229E和猪呼吸道冠状病毒（TGEV的呼吸道变异）中是不存在的。对S1区进行比对，发现了约90个残基的第二结构域，序列差异增加，可能表达病毒特异性决定因子。
The complete sequence of the spike (S) gene of the Br1/87 isolate of porcine epidemic diarrhoea virus (PEDV) was determined from cDNA clones. The predicted polypeptide was 1383 amino acids long, contained 29 potential N-linked glycosylation sites and showed structural features similar to those of the coronavirus spike protein. The PEDV S protein, like that of the members of the transmissible gastroenteritis virus (TGEV)-related subset, lacks a proteolytic site to yield cleaved amino and carboxy subunits S1 and S2. Viral polypeptide species of the expected M(r), i.e. 170K/190K, were observed in PEDV-infected cells. Sequence comparison confirmed that, within the subset, PEDV was most closely related to the human respiratory coronavirus HCV 229E. However, PEDV S protein has an additional 250 residue N-terminal domain which is absent from HCV 229E and porcine respiratory coronavirus, the respiratory variant of TGEV. Alignment of the S1 regions revealed a second domain of about 90 residues with increased sequence divergence which might possibly express virus-specific determinants.