O6-methylguanine DNA methyltransferase activity in diabetic patients
O6-methylguanine DNA methyltransferase activity in diabetic patients
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DOI：
10.1016/s0168-8227(03)00063-9
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发表时间：
2003-07-01
影响因子：
5.1
通讯作者：
Ilkova, H
中科院分区：
文献类型：
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作者：
Akçay, T;Dinçer, Y;Ilkova, H
在本研究中，我们评估了o -6-甲基鸟嘌呤- dna甲基转移酶（MGMT）在糖尿病患者中的活性。该研究对27名1型糖尿病患者和42名2型糖尿病患者进行了研究。有并发症的患者被排除在研究之外。从医务人员中选择36名非糖尿病志愿者，不吸烟，不饮用酒精饮料作为对照。通过将放射性标记的甲基从制备的甲基鸟嘌呤- dna底物转移到白细胞提取物的酶部分来测量MGMT活性。与对照组相比，1型和2型糖尿病患者白细胞MGMT活性显著降低（P < 0.001）。目前的研究表明，1型和2型糖尿病患者白细胞MGMT活性降低。(C) 2003爱思唯尔科学爱尔兰有限公司版权所有。
In the present study, we evaluated O-6-methylguanine-DNA methyltransferase (MGMT) activity in diabetic patients. The study was performed on 27 patients with Type 1 diabetes, and 42 with Type 2 diabetes. Patients with complications were excluded from the study. 36 non-diabetic volunteers, non-smokers who do not consume alcoholic beverage, were chosen from the medical staff as control subjects. MGMT activity was measured by the transfer of radiolabeled methyl groups from a prepared methylguanine-DNA substrate to the enzyme fraction of leukocyte extract. Leukocyte MGMT activity was significantly reduced in both Type 1 and Type 2 diabetes patients as compared with control subjects (P < 0.001). The present study demonstrates decreased MGMT activity in leukocytes from patients with Type 1 and Type 2 diabetes. (C) 2003 Elsevier Science Ireland Ltd. All rights reserved.