Development of synaptic networks in the mouse vagal pathway revealed by optical mapping with a voltage-sensitive dye.
Development of synaptic networks in the mouse vagal pathway revealed by optical mapping with a voltage-sensitive dye.
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通过电压敏感染料的光学测绘揭示了小鼠迷走神经通路中突触网络的发育。
DOI：
10.1111/ejn.13283
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发表时间：
2016
影响因子：
3.4
通讯作者：
K.
中科院分区：
文献类型：
--
作者：
Momose-Sato;Y. and Sato;K.
发育神经科学的中心问题是神经突触网络何时以及如何在中枢神经系统(CNS)内建立并发挥作用。由于传统的电生理手段具有一定的技术局限性，神经网络组织的研究一直受到阻碍。本研究采用电压敏感染料多点光学记录技术，对小鼠胚胎迷走神经系统的发育组织进行了研究。刺激E11-E14小鼠胚胎的迷走神经在迷走神经感觉和运动核团对应的区域引起光学反应。第一级感觉核-孤束核(NTS)的突触后反应从E11开始被发现，提示感觉信息在这个阶段被转移到大脑。除NTS外，在吻侧和对侧脑干区也有光学反应，与迷走神经的二阶/高阶核团相对应，包括臂旁核(PBN)。在E12的第二/高级核团中检测到突触后反应，表明多突触网络在这个阶段是有功能的。我们讨论了我们的光学图谱的结果，并将它们与以前在鸡和大鼠胚胎中获得的结果进行了比较，并提出了胚胎大脑中突触网络功能组织的一些基本原则。
The central issue in developmental neuroscience is when and how neural synaptic networks are established and become functional within the central nervous system (CNS). Investigations of the neural network organization have been hampered because conventional electrophysiological means have some technical limitations. In this study, the multiple‐site optical recording technique with a voltage‐sensitive dye was employed to survey the developmental organization of the vagal system in the mouse embryo. Stimulation of the vagus nerve in E11–E14 mouse embryos elicited optical responses in areas corresponding to the vagal sensory and motor nuclei. Postsynaptic responses in the first‐order sensory nucleus, the nucleus of the tractus solitarius (NTS), were identified from E11, suggesting that sensory information becomes transferred to the brain at this stage. In addition to the NTS, optical responses were identified in the rostral and contralateral brainstem regions, which corresponded to second/higher order nuclei of the vagus nerve including the parabrachial nucleus (PBN). Postsynaptic responses in the second/higher‐order nuclei were detected from E12, suggesting that polysynaptic networks were functional at this stage. We discuss the results of our optical mapping, comparing them with previous findings obtained in the chick and rat embryos, and suggest some fundamental principles in the functional organization of synaptic networks in the embryonic brain.