Neutrophils have a protective role during early stages of Leishmania amazonensis infection in BALB/c mice.
Neutrophils have a protective role during early stages of Leishmania amazonensis infection in BALB/c mice.
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DOI：
10.1111/pim.12078
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发表时间：
2014-01
影响因子：
2.2
通讯作者：
Vieira LQ
中科院分区：
文献类型：
--
作者：
Sousa LM;Carneiro MB;Resende ME;Martins LS;Dos Santos LM;Vaz LG;Mello PS;Mosser DM;Oliveira MA;Vieira LQ
中性粒细胞参与对病原体的免疫应答的早期阶段。在这里，我们调查的作用，中性粒细胞在建立利什曼原虫亚马逊感染BALB/c和C57 BL/6小鼠。首先，我们发现中性粒细胞在感染后6至24小时之间积聚，随后在72小时后中性粒细胞数量减少。接下来，我们在感染前使用RB 6 - 8 C5或1A 8 mAb耗尽嗜中性粒细胞。在感染的第一周内，中性粒细胞消耗导致BALB/c小鼠的病变发展更快、寄生虫数量增加和精氨酸酶活性更高，但在C57 BL/6小鼠中则不然。易感性增加伴随着抗-L水平的增加。amazonensis IgG和增加的IL-10和IL-17的产生。由于IL-10是利什曼原虫感染易感性的介体，我们使用抗IL-10 R阻断了嗜利什曼原虫耗竭小鼠中的IL-10信号传导。有趣的是，IL-10信号传导的抑制消除了在嗜中性粒细胞耗竭小鼠中观察到的寄生虫负荷的增加，表明寄生虫增殖至少部分由IL-10介导。此外，我们测试了IL-17在炎性巨噬细胞中的作用，并观察到IL-17增加了巨噬细胞酶活性并有利于寄生虫生长。总之，我们的数据表明，中性粒细胞控制寄生虫的数量和限制病变的发展，在第一周的感染BALB/c小鼠。
Neutrophils are involved in the early stages of immune responses to pathogens. Here, we investigated the role of neutrophils during the establishment of Leishmania amazonensis infection in BALB/c and C57BL/6 mice. First, we showed an accumulation of neutrophils between 6 and 24 h post-infection, followed by a reduction in neutrophil numbers after 72 h. Next, we depleted neutrophils prior to infection using RB6-8C5 or 1A8 mAb. Neutrophil depletion led to faster lesion development, increased parasite numbers and higher arginase activity during the first week of infection in BALB/c mice, but not in C57BL/6 mice. Increased susceptibility was accompanied by augmented levels of anti-L. amazonensis IgG and increased production of IL-10 and IL-17. Because IL-10 is a mediator of susceptibility to Leishmania infection, we blocked IL-10 signalling in neutrophil-depleted mice using anti-IL-10R. Interestingly, inhibition of IL-10 signalling abrogated the increase in parasite loads observed in neutrophil-depleted mice, suggesting that parasite proliferation is at least partially mediated by IL-10. Additionally, we tested the effect of IL-17 in inflammatory macrophages and observed that IL-17 increased arginase activity and favoured parasite growth. Taken together, our data indicate that neutrophils control parasite numbers and limit lesion development during the first week of infection in BALB/c mice.
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影响因子：
5.8
作者：
Gomes, IN;Calabrich, AFD;Veras, PST
通讯作者：
Veras, PST
DOI：
10.1084/jem.169.1.59
发表时间：
1989-01-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Heinzel FP;Sadick MD;Holaday BJ;Coffman RL;Locksley RM
通讯作者：
Locksley RM
影响因子：
5.5
作者：
Afonso, Lilian;Borges, Valeria M.;Brodskyn, Claudia I.
通讯作者：
Brodskyn, Claudia I.
影响因子：
3.1
作者：
AFONSO, LCC;SCOTT, P
通讯作者：
SCOTT, P
影响因子：
4.4
作者：
Bennouna, S;Bliss, SK;Denkers, EY
通讯作者：
Denkers, EY