Sequencing and promoter analysis of the genomic region between the rat neuronal nicotinic acetylcholine receptor beta 4 and alpha 3 genes.
Sequencing and promoter analysis of the genomic region between the rat neuronal nicotinic acetylcholine receptor beta 4 and alpha 3 genes.
复制标题
大鼠神经元烟碱乙酰胆碱受体β4和α3基因之间的基因组区域的测序和启动子分析。
DOI：
10.1002/neu.480250806
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Boyd,RT
中科院分区：
文献类型：
--
作者：
Boyd,RT
Nicotinic acetylcholine receptors (nAChRs) found on neurons are composed of ligand binding (α) and structural (β) subunits. Different combinations of α and β subunits produce nAChR subtypes with different pharmacological and ion‐conducting proeprties. Transcriptional regulation may be an important determinant of receptor subtype in a neuronal population and thus influence transmission through a ganglion or group of neurons in the CNS by controlling the nAChR subtype (s) present. In order to understand the transcriptional regulation of neuronal nAChRs by cell contact and electrical activity, it will be first necessary to identify DNA elements that control the expression of memebrs of this family and to identify factors required for the expression of these genes. In this report we have begun to examine the 5′‐ flanking region of one member of the nAChR family of genes, α3. We have sequenced the region between the β4 and α3 genes and have identified two promoter regions in the β4‐α3 intergenic region. One region is close to the β4 gene downstream of exon 6 and has strong promoter activity in both orientations; the other is close to the start of the α3 gene coding region. A region with putative silencer acitivity is also found near the upstream promoter. This bidirectional promoter region could be involved in the control of α3 and β4 gene expression. 1994 John Wiley & Sons, Inc.
