Neurotensin: a modulator of enteric cholinergic neurons in the guinea pig small intestine.
Neurotensin: a modulator of enteric cholinergic neurons in the guinea pig small intestine.
复制标题
神经降压素：豚鼠小肠肠胆碱能神经元的调节剂。
DOI：
10.1016/0014-2999(83)90642-8
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发表时间：
1983
影响因子：
5
通讯作者：
Youther,ML
中科院分区：
文献类型：
--
作者：
Yau,WM;Verdun,PR;Youther,ML
本文研究了神经降压素（NT）存在时豚鼠小肠肌间神经丛-纵肌条（MPLM）上[~ 3 H]乙酰胆碱的释放。在3 pM至3 nM的剂量范围内，NT诱导[3 H]乙酰胆碱从MPLM流出的剂量相关性增加。这种释放被1.5 × 10− 6 M河豚毒素完全抑制。NT（7.5 × 10− 11 M）诱发的乙酰胆碱输出量被添加生长抑素（6.1 × 10− 7 M）或甲硫氨酸脑啡肽（8.7 × 10− 8 M）显著抑制。在NT（31.5 × 10 - 7 M）或20 mM KCl存在下，还检测了分离的突触末梢（突触体）中新合成的[3 H]乙酰胆碱的释放。[3 H]乙酰胆碱的释放显着升高，无论是兴奋剂。结果表明，神经降压素通过神经原性机制刺激乙酰胆碱释放，可能是通过肌间胆碱能神经元的突触前作用位点。尽管肌间神经丛中缺乏含NT的神经元，但本研究的结果支持NT在调节肠道局部胆碱能神经元活动中的神经调节作用。
Release of [3H]acetylcholine was studied in the presence of neurotensin (NT) on the myenteric plexus-longitudinal muscle strips (MPLM) from the guinea pig small intestine. In the dose range from 3 pM to 3 nM, NT induced a dose-related increase in the efflux of [3H]acetylcholine from the MPLM. This release was completely inhibited by 1.5 × 10−6M tetrodotoxin. The output of acetylcholine evoked by NT (7.5 × 10−11M) was significantly depressed by the addition of somatostatin (6.1 ¢ 10−7M) or methionine-enkephalin (8.7 × 10−8M). Release of newly synthesized [3H]acetylcholine from the isolated synaptic terminals (synaptosomes) was also examined in the presence of NT 31.5 ¢ 10−7M) or 20 mM KCl. Release of [3H]acetylcholine was significantly elevated by either stimulants. It was concluded that neurotensin stimulated ACh release via a neurogenic mechanism, probably by a presynaptic site of action on myenteric cholinergic neurons. Despite of a paucity of NT-containing neurons in the myenteric plexus, results from this styd support a neuromodulatory role for NT in the regulation of local cholinergic neuronal activity in the gut.