Discovery and validation of peptide biomarkers for discrimination of Dendrobium species by label-free proteomics and chemometrics
Discovery and validation of peptide biomarkers for discrimination of Dendrobium species by label-free proteomics and chemometrics
复制标题
通过无标记蛋白质组学和化学计量学发现和验证用于区分石斛兰属物种的肽生物标志物
DOI：
10.1016/j.jpba.2020.113118
复制
发表时间：
2020-04-15
影响因子：
3.4
通讯作者：
Zhou, Jian-Liang
中科院分区：
文献类型：
--
作者：
Fang, Can;Xin, Gui-Zhong;Zhou, Jian-Liang
铁皮石斛是亚洲著名的名贵食品和中草药，但近年来却因使用价格较低、易混淆的石斛属植物如铁皮石斛而遭受掺假和假冒。devonianum或D.草药市场的卖药人。然而，目前还没有可靠的方法来鉴定D. officinale从其易混淆的物种有效的仍然缺乏，特别是干燥样品。本研究致力于寻找特异性肽类生物标志物，用于D.从其他两种石斛属物种中使用nanoLC LTQ Orbitrap质谱法的无标记蛋白质组学鉴定铁皮石斛。多元统计分析，以可视化之间的差异，三种石斛。结果，从343种可测肽中筛选出29种肽作为石斛属植物分类的潜在生物标志物。利用合成的肽段对代表性肽段生物标志物进行验证，发现3个肽段生物标志物对D. officinale。进一步的分析表明，肽ALGLELDLSER也可能是区分D.原产于不同的地理区域。(C)2020爱思唯尔B.V.保留所有权利。
The stems of Dendrobium officinale, a well-known and expensive food material and herbal medicine in Asia, has recently suffered adulterants and counterfeits by using lower-price confusing Dendrobium species such as D. devonianum or D. transparens in the herbal market. However, robust methods that could authenticate D. officinale from its confusing species effectively are still lacking, especially for the dried samples. This study committed to discover specific peptides biomarkers for the authentication of D. officinale from the other two Dendrobium species using label-free proteomics by nanoLC LTQ Orbitrap mass spectrometry. Multivariate statistical analysis was applied to visualize the difference between the three Dendrobium species. As a result, 29 peptides among a total of 343 measurable peptides were selected to be potential biomarkers for the classification of these Dendrobium species. The validation of the representative peptide biomarkers was carried out by the synthesized peptides and 3 peptide biomarkers were found significant for the authentication of D. officinale. Further analysis showed that peptide ALGLELDLSER may also be a biomarker for the discrimination of the D. officinale originated from different geographical regions. (C) 2020 Elsevier B.V. All rights reserved.