Improvement of Measurement Method for Superoxide Anion Radical in Plant
Improvement of Measurement Method for Superoxide Anion Radical in Plant
复制标题
DOI：
--
复制
发表时间：
2005
期刊：
影响因子：
--
通讯作者：
L. Zhong
中科院分区：
文献类型：
--
作者：
L. Zhong
分析了超氧阴离子自由基与羟胺反应的动力学曲线，确定了最佳反应介质、反应参数和羟胺浓度，结果表明，用化学试剂氯仿代替乙醚作为植物色素的提取剂，克服了植物体内超氧阴离子自由基测定方法存在的一些问题，提高了测定的准确性、重现性和可比性。
This paper analysed kinetics curve of superoxide anion radical reacting with hydroxylamine.Optimum reaction medium,reaction parameters and hydroxylamine concentration were confirmed.The result indicated that chemical reagent chloroform instead of ethyl ether as extraction reagent of plant pigment,overcomed some problems of measurement method for superoxide anion radical in plant,and enhanced the accuracy,reproducibility and comparability.