QUANTITATIVE ANALYSIS OF UNTREATED HUMAN NAILS FOR MONITORING HUMAN EXPOSURE TO HEAVY METALS
QUANTITATIVE ANALYSIS OF UNTREATED HUMAN NAILS FOR MONITORING HUMAN EXPOSURE TO HEAVY METALS
复制标题
对未经处理的人类指甲进行定量分析，以监测人类接触重金属的情况
DOI：
10.1142/s0129083502000226
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
E. Clemente
中科院分区：
文献类型：
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作者：
K. Sera;S. Futatsugawa;S. Murao;E. Clemente
为了解决全球环境问题，我们开发了一种无标准方法，已成功地应用于各种生物样品。特别是，一种未经处理的头发的方法已被应用于许多污染地区，以研究人类暴露于有毒元素。除了头发，指甲有望为我们提供有关人类接触有毒元素的宝贵信息。然而，分析需要相对大量的样品和费力的样品制备技术，这需要内标。在这项工作中，我们已经开发了一种定量的方法，未经处理的人指甲分析的基础上，标准的方法。它既不需要大量的钉子，也不需要复杂的目标准备过程。此外，它完全没有目标制备中的任何模糊性，例如化学灰化过程中某些元素的挥发和样品的污染。确定了指甲样品辐照的最佳条件，并证实了本方法的准确性和重现性。结果发现，在蒸馏水中的超声波清洗是有效的许多指甲样品，防止从样品中的元素的损失。还发现，元素浓度的指甲强烈地依赖于他们的采样位置。
In order to address global environmental issues, a standard-free method developed by ourselves has been successfully applied to various kinds of bio-samples. Especially, a method for untreated hairs has been applied in many polluted areas to study human exposure to toxic elements. In addition to hair, nail is expected to give us valuable information about human exposure to toxic elements. However, the analysis requires relatively large amounts of samples and laborious sample preparation techniques which necessitate internal standards. In this work, we have developed a quantitative method for untreated human-nail analysis based on the standard-free method. It requires neither large amounts of nails nor complicated target preparation procedure. Furthermore, it is perfectly free from any ambiguity in target preparation such as volatilization of certain elements and contamination of the sample during chemical ashing. The optimum conditions of irradiating nail samples are established, and accuracy and reproducibility of the present method are confirmed. It is found that ultrasonic washing in distilled water is effective for many nail samples preventing the loss of elements from the sample. It is also found that elemental concentration in nails strongly depends on their sampling positions.