Physiological function of S-cone system is not enhanced in rd7 mice
Physiological function of S-cone system is not enhanced in rd7 mice
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DOI：
10.1016/j.exer.2005.04.013
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发表时间：
2005-12-01
影响因子：
3.4
通讯作者：
Miyake, Y
中科院分区：
文献类型：
--
作者：
Ueno, S;Kondo, M;Miyake, Y
Rd7小鼠是视网膜变性发展相对较晚的突变小鼠。早期的研究表明，RD7小鼠具有独特的视网膜发育不良模式，锥体细胞数量增加，特别是那些具有S(短波)-视蛋白免疫反应的小鼠。RD7视网膜的这些变化是由光感受器细胞特异性核受体基因Nr2e3突变引起的，Nr2e3参与调节光感受器细胞分化、细胞维持和细胞-细胞相互作用的信号通路。本研究的目的是利用视网膜电信号(ERG)技术来确定RD7视网膜的生理特性。我们发现RD7小鼠视网膜电信号的最大a波幅在6周龄时已经降低到同种对照的一半，并具有正常的光传导敏感性。RD7组小鼠的明视ERG无异常，S视锥ERG的波幅与对照组无显著差异。这些结果表明，即使表达S视蛋白的视锥细胞数目增加，但S视锥系统的生理功能并未得到增强。(C)2005爱思唯尔有限公司。保留所有权利。
The rd7mouse is a mutant mouse with a relatively late development of retinal degeneration. Earlier studies have shown that rd7 mice have a distinctive pattern of retinal dysplasia with an increased number of cone cells, particularly those with S (short wavelength)-opsin immunoreactivity. These alterations of the rd7 retina are caused by a mutation in the photoreceptor cell-specific nuclear receptor gene, Nr2e3, which is involved in the signaling pathway regulating photoreceptor cell differentiation, cell maintenance, and cell-cell interactions. The purpose of this study was to determine the physiological properties of the rd7 retina using electroretinographic (ERG) techniques. We found that the maximal a-wave amplitude of the ERG in rd7 mice was already reduced to half of the congenic controls at 6 weeks of age with normal phototransduction sensitivity. The photopic ERGs of rd7 mice were not supernormal, and the amplitudes of the S-cone ERGs were not significantly different from those recorded in controls. These results suggested that even though the number of cones expressing S-opsin is increased, the physiological function of the S-cone system is not enhanced in rd7 mice. (c) 2005 Elsevier Ltd. All rights reserved.