Permanent genetic memory with >1-byte capacity
Permanent genetic memory with >1-byte capacity
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DOI：
10.1038/nmeth.3147
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发表时间：
2014-12-01
期刊：
影响因子：
48
通讯作者：
Voigtwill, Christopher A.
中科院分区：
文献类型：
--
作者：
Yang, Lei;Nielsen, Alec A. K.;Voigtwill, Christopher A.
遗传记忆使得能够在活细胞的DNDNA中记录信息。存储器可以记录瞬态环境信号或细胞状态，然后在稍后的时间调用。永久性记忆是使用不可逆重组酶实现的，这些重组酶反转DNDNA单元的方向，对应于位的[0，1]状态。为了扩大记忆容量，我们应用生物信息学来鉴定34种噬菌体整合酶（及其同源attB和attP识别位点），从中我们构建了11种彼此完全正交的记忆开关以及FimE和HbiF细菌转化酶。使用这些开关，在大肠杆菌中构建了一个存储器阵列，可以记录1.375字节的信息。它表明，重组酶可以分层，并用于永久记录转录逻辑门的瞬态。
Genetic memory enables the recording of information in the DNDNA of living cells. Memory can record a transient environmental signal or cell state that is then recalled at a later time. Permanent memory is implemented using irreversible recombinases that invert the orientation of a unit of DNDNA, corresponding to the [0,1] state of a bit. To expand the memory capacity, we have applied bioinformatics to identify 34 phage integrases (and their cognate attB and attP recognition sites), from which we build 11 memory switches that are perfectly orthogonal to each other and the FimE and HbiF bacterial invertases. Using these switches, a memory array is constructed in Escherichia coli that can record 1.375 bytes of information. It is demonstrated that the recombinases can be layered and used to permanently record the transient state of a transcriptional logic gate.