Augmented DNA Nanoarchitectures: A Structural Library of 3D Self‐Assembling Tensegrity Triangle Variants
Augmented DNA Nanoarchitectures: A Structural Library of 3D Self‐Assembling Tensegrity Triangle Variants
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增强型 DNA 纳米结构：3D 自组装张拉整体三角形变体的结构库
DOI：
10.1002/adma.202206876
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发表时间：
2022
影响因子：
29.4
通讯作者：
Jonoska, Nataša
中科院分区：
文献类型：
--
作者：
Woloszyn, Karol;Vecchioni, Simon;Ohayon, Yoel P.;Lu, Brandon;Ma, Yinglun;Huang, Qiuyan;Zhu, Eric;Chernovolenko, Daniel;Markus, Tiffany;Jonoska, Nataša
众所周知，DNA 张拉整体三角形可以通过粘端内聚力可靠地自组装成 3D 菱形晶格。在这里，可访问的图案库通过三角形间区域的共价延伸以及使用几何对称和不对称配置的相邻三角形与双螺旋段的粘性末端协调连接来扩展。报告了 18 种分辨率为 3.32-9.32 Å 的自组装结构的分子结构；观察到的细胞尺寸、空腔尺寸和横截面积与理论预期一致。这些数据表明，通过合理设计可以实现对三斜晶和菱面体晶体参数的精细控制以及更复杂的 3D DNA 晶格的可定制性。预计增强的 DNA 架构可能会像最初设想的那样，针对设计纳米笼、客体复合物和限制性 3D 纳米材料的自组装进行微调。最后，设计者的不对称晶体构建块可以被视为控制和编码三维信息的第一步。
The DNA tensegrity triangle is known to reliably self‐assemble into a 3D rhombohedral crystalline lattice via sticky‐end cohesion. Here, the library of accessible motifs is expanded through covalent extensions of intertriangle regions and sticky‐end‐coordinated linkages of adjacent triangles with double helical segments using both geometrically symmetric and asymmetric configurations. The molecular structures of 18 self‐assembled architectures at resolutions of 3.32–9.32 Å are reported; the observed cell dimensions, cavity sizes, and cross‐sectional areas agree with theoretical expectations. These data demonstrate that fine control over triclinic and rhombohedral crystal parameters and the customizability of more complex 3D DNA lattices are attainable via rational design. It is anticipated that augmented DNA architectures may be fine‐tuned for the self‐assembly of designer nanocages, guest–host complexes, and proscriptive 3D nanomaterials, as originally envisioned. Finally, designer asymmetric crystalline building blocks can be seen as a first step toward controlling and encoding information in three dimensions.