COMPETITIVE ENZYME IMMUNOASSAYS FOR THE RAPID DETECTION OF ANTIBODIES TO FELINE INFECTIOUS PERITONITIS VIRUS POLYPEPTIDES
COMPETITIVE ENZYME IMMUNOASSAYS FOR THE RAPID DETECTION OF ANTIBODIES TO FELINE INFECTIOUS PERITONITIS VIRUS POLYPEPTIDES
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DOI：
10.1128/jcm.22.3.395-401.1985
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发表时间：
1985-01-01
影响因子：
9.4
通讯作者：
PEDERSEN, NC
中科院分区：
文献类型：
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作者：
FISCUS, SA;TERAMOTO, YA;PEDERSEN, NC
用抗猫传染性腹膜炎病毒（FIPV）包膜多肽（E1）、peplomer多肽（E2）和核衣壳多肽（N）的单克隆抗体进行快速竞争性酶联免疫吸附试验（ELISA），研究猫对FIPV感染的体液免疫应答。从竞争ELISA的结果与免疫荧光抗体测定（IFA）的203个样品从64只猫。IFA结果与抗E1特异性竞争ELISA结果的相关性最好（85.7%）。抗-N和抗-E2竞争性ELISA结果与IFA结果的相关性分别为65.5%和2.4%。抗E1特异性竞争ELISA的结果不受总IG浓度或血清中可能存在的游离病毒抗原的影响。这些结果表明，竞争性ELISA涉及使用酶偶联的单克隆抗体的E1糖蛋白的FIPV是一个简单和快速的替代更繁琐的IFA。
Monoclonal antibodies specific for the envelope (E1), peplomer (E2), and nucleocapsid (N) polypeptides of feline infectious peritonitis virus (FIPV) were used in rapid, competitive enzyme-linked immunosorbent assays (ELISA) to study the humoral immune response of cats to FIPV infection. Results from the competitive ELISA were correlated with those from immunofluorescent antibody assays (IFA) on 203 samples obtained from 64 individual cats. The IFA results correlated best with those obtained with the anti-E1 specific competitive ELISA (85.7%). Anti-N and anti-E2 competitive ELISA results correlated with IFA results only 65.5 and 2.4% of the time, respectively. The results of the anti-E1 specific competitive ELISA were not influenced by the total Ig concentration or the possible presence of free viral antigens in the serum. These results suggest that a competitive ELISA involving the use of enzyme-conjugated monoclonal antibody to the E1 glycoprotein of FIPV is a simple and rapid replacement for the more cumbersome IFA.