Arabidopsis subtilase SASP is involved in the regulation of ABA signaling and drought tolerance by interacting with OPEN STOMATA 1
Arabidopsis subtilase SASP is involved in the regulation of ABA signaling and drought tolerance by interacting with OPEN STOMATA 1
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DOI：
10.1093/jxb/ery205
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发表时间：
2018-06
影响因子：
6.9
通讯作者：
Qianqian Wang;Q. Guo;Yuanyuan Guo;Jieshu Yang;Min Wang;Xiaoke Duan;Jiayu Niu;Shuai Liu;Jianzhen Zhang;Yanke Lu;Zhi-hui Hou;W. Miao;Xiangyu Wang;Weiwen Kong;Xiaoming Xu;Yufeng Wu;Q. Rui;Honggui La
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文献类型：
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作者：
Qianqian Wang;Q. Guo;Yuanyuan Guo;Jieshu Yang;Min Wang;Xiaoke Duan;Jiayu Niu;Shuai Liu;Jianzhen Zhang;Yanke Lu;Zhi-hui Hou;W. Miao;Xiangyu Wang;Weiwen Kong;Xiaoming Xu;Yufeng Wu;Q. Rui;Honggui La
先前报道拟南芥衰老相关枯草杆菌蛋白酶（SASP）参与叶片衰老以及花序和长角果的发育。在这里，我们描述了 SASP 在脱落酸 (ABA) 信号传导调节中的新作用。 SASP 编码枯草杆菌酶，黑暗、ABA 和乙烯处理可显着诱导其表达。 sasp 敲除突变体表现出明显的发育表型，例如早花和较小的叶子。特别是，sasp 突变体在种子萌发和幼苗生长过程中表现出增强的 ABA 敏感性，增强了 ABA 介导的叶片衰老，并增加了活性氧 (ROS) 的产生。重要的是，sasp 突变体还表现出对干旱的耐受性显着增强，六个 ABA 信号相关基因的表达在 ABA 处理后上调或下调。相互作用测定表明，SASP 与细胞外围的 OPEN STOMATA 1 (OST1) 发生物理相互作用。 SASP 和 OST1 的共表达导致 OST1 降解，而这种降解在 sasp-1 原生质体中受到损害。 ROS衰减测定表明，在sasp-1突变体保卫细胞中，衰减率显着降低。综上所述，结果表明 SASP 通过与 OST1 相互作用在调节 ABA 信号传导和耐旱性方面发挥着重要作用。
Arabidopsis Senescence-Associated Subtilisin Protease (SASP) has previously been reported to participate in leaf senescence and in the development of inflorescences and siliques. Here, we describe a new role of SASP in the regulation of abscisic acid (ABA) signaling. SASP encodes a subtilase and its expression was considerably induced by darkness, ABA, and ethylene treatments. sasp knockout mutants displayed obvious developmental phenotypes such as early flowering and smaller leaves. In particular, the sasp mutants exhibited enhanced ABA sensitivity during seed germination and seedling growth, heightened ABA-mediated leaf senescence, and increased production of reactive oxygen species (ROS). Importantly, the sasp mutants also showed remarkably increased tolerance to drought, with expression of six ABA signaling-related genes being either up- or down-regulated following ABA treatment. Interaction assays demonstrated that SASP physically interacts with OPEN STOMATA 1 (OST1) at the cell periphery. Co-expression of SASP and OST1 led to degradation of OST1, whereas this degradation was impaired in sasp-1 protoplasts. ROS attenuation assays demonstrated that in sasp-1 mutant guard cells the attenuation rate markedly decreased. Taken together, the results demonstrate that SASP plays an important role in regulating ABA signaling and drought tolerance through interaction with OST1.