Silver nanoparticles as matrix for laser desorption/ionization mass spectrometry of peptides
Silver nanoparticles as matrix for laser desorption/ionization mass spectrometry of peptides
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DOI：
10.1007/s11051-007-9244-4
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发表时间：
2007-12-01
影响因子：
2.5
通讯作者：
Tan, Swee Ngin
中科院分区：
文献类型：
--
作者：
Hua, Lin;Chen, Jianrong;Tan, Swee Ngin
利用化学还原法制备的纳米银作为基质，成功地用于多肽的基质辅助激光解吸电离飞行时间质谱仪(MALDI-TOF-MS)。纳米银作为吸附分析物的底物和紫外光的传输介质，被发现有助于多肽的解吸/电离，而诱导的碎裂很少或没有诱导碎裂。纳米颗粒的尺寸通常在160+/-20 nm的范围内。银纳米粒子用于多肽分析的关键优点之一是其在探针样品制备步骤简单。此外，与α-氰基-4-羟基肉桂酸(CCA)基质相比，它还最大限度地减少了十二烷基硫酸钠(SDS)表面活性剂背景信号的干扰，获得了更清晰的质谱图和更灵敏的信号。
Silver nanoparticle synthesized from chemical reduction has been successfully utilized as a matrix in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) of peptides. Acting as a substrate to adsorb analytes, as well as a transmission medium for UV laser, silver nanoparticle was found to assist in the desorption/ionization of peptides with little or no induced fragmentation. The size of the nanoparticle was typically in the range of 160 +/- 20 nm. One of the key advantages of silver nanoparticle for peptides analysis is its simple step for on-probe sample preparation. In addition, it also minimizes the interferences of sodium dodecyl sulfate (SDS) surfactant background signal, resulting in cleaner mass spectra and more sensitive signal, when compared to alpha-cyano-4-hydroxycinnamic acid (CCA) matrix.