Interspecies Competition Impacts Targeted Manipulation of Human Gut Bacteria by Fiber-Derived Glycans
Interspecies Competition Impacts Targeted Manipulation of Human Gut Bacteria by Fiber-Derived Glycans
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DOI：
10.1016/j.cell.2019.08.011
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发表时间：
2019-09-19
期刊：
影响因子：
64.5
通讯作者：
Gordon, Jeffrey I.
中科院分区：
文献类型：
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作者：
Patnode, Michael L.;Beller, Zachary W.;Gordon, Jeffrey I.
开发微生物导向食品（MDFs），选择性地增加有益的人类肠道微生物的丰度及其表达的功能，需要了解MDFs的生物活性成分和微生物-微生物相互作用的机制。在这里，gnotobiotic小鼠被人类肠道来源的细菌菌株的定义财团定殖，并喂食添加到美国消费的代表性低纤维饮食中的34种食品级纤维的不同组合。纤维制剂中的生物活性碳水化合物靶向特定拟杆菌属物种的鉴定，使用细菌基因表达的全社区定量蛋白质组学分析加上正向遗传筛选。故意操纵的社区成员相结合的管理可检索的人工食品颗粒，由顺磁性微观珠涂有膳食多糖，披露的贡献，目标物种的纤维降解。我们的方法，包括使用珠为基础的生物传感器，定义的营养收获策略的基础，以及减轻，类杆菌之间的竞争和控制的选择性的细菌组分。
Development of microbiota-directed foods (MDFs) that selectively increase the abundance of beneficial human gut microbes, and their expressed functions, requires knowledge of both the bioactive components of MDFs and the mechanisms underlying microbe-microbe interactions. Here, gnotobiotic mice were colonized with a defined consortium of human-gut-derived bacterial strains and fed different combinations of 34 food-grade fibers added to a representative low-fiber diet consumed in the United States. Bioactive carbohydrates in fiber preparations targeting particular Bacteroides species were identified using community-wide quantitative proteomic analyses of bacterial gene expression coupled with forward genetic screens. Deliberate manipulation of community membership combined with administration of retrievable artificial food particles, consisting of paramagnetic microscopic beads coated with dietary polysaccharides, disclosed the contributions of targeted species to fiber degradation. Our approach, including the use of bead-based biosensors, defines nutrient-harvesting strategies that underlie, as well as alleviate, competition between Bacteroides and control the selectivity of MDF components.