The complete mitochondrial DNA genome of Eremias brenchleyi (Reptilia: Lacertidae) and its phylogeny position within squamata reptiles
The complete mitochondrial DNA genome of Eremias brenchleyi (Reptilia: Lacertidae) and its phylogeny position within squamata reptiles
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DOI：
10.1163/156853809787392793
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发表时间：
2009
影响因子：
1.6
通讯作者：
L. Nie;Yuting Wang;Jin-Long Rui
中科院分区：
文献类型：
--
作者：
L. Nie;Yuting Wang;Jin-Long Rui
有鳞目是最多样化的爬行动物目，传统上分为三个亚目- Lacertilia，Serpentes和Amphisbaenia，其中Lacertilia约有16-19个科。但蜥蜴目主要类群之间的系统发育关系仍存在争议。本文采用长片段精确PCR（LA-PCR）技术，测定了渤海沙蚕线粒体DNA的全序列。线粒体DNA序列长19542 bp，是目前报道的有鳞类线粒体基因组中最长的。它显示了典型的脊椎动物基因排列。E. brendezyi的5′端和3′端分别有65 bp和56 bp的串联重复序列。为了研究有鳞类的更高层次的亲缘关系，本研究对现有的所有有鳞类线粒体序列进行了系统发育研究。通过对16科蜥蜴（蜥蜴科、石鸡科、鬣蜥科、变色龙科、鬣蜥科、刺角蜥科、双足蜥科、Shinisauridae、Helodermatidae、Amphisbaenidae、壁虎科、Varanidae、Anguidae、Xantusiidae、Rhineuridae、Cordylidae）和8科蛇的亲缘关系的研究，初步确定了蜥蜴类的亲缘关系。该组内的内部关系产生了高的自助支持，更符合形态分析。
Squamata is the most diversified reptilian order that has been traditionally classified into three suborders – Lacertilia, Serpentes and Amphisbaenia in which Lacertilia have about 16-19 families. But the phylogenetic relationships among major groups of Lacertilia remain controversial. In this paper, the complete mitochondrial DNA sequence of Eremias brenchleyi was determined by using long-and-accurate PCR (LA-PCR). The mtDNA sequence is 19 542 bp, making it the longest mitochondrial genome in squamates species reported so far. It shows the typical vertebrate arrangement of genes. The control region of E. brenchleyi was characterized by two conspicuous 65 bp and 56 bp tandem repeats at its 5′ and 3′ terminus respectively. In order to study the higher level relationships of squamates, the phylogenetic study including all currently available squamates mitochondrial sequences was carried out. We obtained a relationship of 16 families of lizards (Lacertidae, Scincidae, Iguanidae, Chameleonidae, Agamidae, Trogonophidae, Bipedidae, Shinisauridae, Helodermatidae, Amphisbaenidae, Gekkonidae, Varanidae, Anguidae, Xantusiidae, Rhineuridae, Cordylidae) and 8 families of Serpentes. The internal relationships within this group yielded high bootstrap support and were more congruent with morphological analyses.