The mitochondrial cytochrome c N-terminal region is critical for maturation by holocytochrome c synthase.
The mitochondrial cytochrome c N-terminal region is critical for maturation by holocytochrome c synthase.
复制标题
线粒体细胞色素 c N 末端区域对于全细胞色素 c 合酶的成熟至关重要。
DOI：
10.1016/j.febslet.2011.04.058
复制
发表时间：
2011
期刊：
影响因子：
3.5
通讯作者：
Stevens JM
中科院分区：
文献类型：
--
作者：
Stevens JM
血红素与线粒体细胞色素的共价结合是由全细胞色素合成酶（HCCS，也称血红素裂解酶）催化的。HCCS如何起作用和识别底物脱辅基细胞色素尚不清楚。在这里，我们已经研究了HCCS识别嵌合基板包括一个短的线粒体cytochromecN-末端区域的C-末端序列，包括CXXCH血红素结合基序，细菌cytochromecthat不是否则由HCCS处理。血红素连接的嵌合体证明了N-末端区域的细胞色素的重要性。一系列的线粒体细胞色素的变异体与氨基酸替换的N-末端区域缩小了特异性决定簇，提供了深入了解HCCS底物识别。
The covalent attachment of heme to mitochondrial cytochromecis catalysed by holocytochromecsynthase (HCCS, also called heme lyase). How HCCS functions and recognises the substrate apocytochrome is unknown. Here we have examined HCCS recognition of a chimeric substrate comprising a short mitochondrial cytochromecN-terminal region with the C-terminal sequence, including the CXXCH heme-binding motif, of a bacterial cytochromecthat is not otherwise processed by HCCS. Heme attachment to the chimera demonstrates the importance of the N-terminal region of the cytochrome. A series of variants of a mitochondrial cytochromecwith amino acid replacements in the N-terminal region have narrowed down the specificity determinants, providing insight into HCCS substrate recognition.