Chromosomal instability is a risk factor for poor prognosis of adenocarcinoma of the lung: Fluorescence in situ hybridization analysis of paraffin-embedded tissue from Korean patients
Chromosomal instability is a risk factor for poor prognosis of adenocarcinoma of the lung: Fluorescence in situ hybridization analysis of paraffin-embedded tissue from Korean patients
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DOI：
10.1016/j.lungcan.2008.07.016
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发表时间：
2009-04-01
期刊：
影响因子：
5.3
通讯作者：
Lee, Sang-Do
中科院分区：
文献类型：
--
作者：
Choi, Chang-Min;Seo, Kwang Won;Lee, Sang-Do
背景：在本研究中，我们试图评估染色体不稳定性（CIN）在肺腺癌（AC）预后中的重要性。荧光原位杂交（FISH）检测CIN与AC患者生存的关系。方法：对63例肺AC手术标本进行分析。对石蜡包埋的肿瘤组织中提取的细胞核进行c-myc、6号染色体、EGFR和5号染色体（LAVysion、Vysis）的多靶点DNA FISH检测，用CIN、p16和多靶点DNA FISH检测肿瘤。生存率根据性别、年龄、组织学、T因子、N因子、CIN和吸烟状况进行比较。如果一个样本的5条染色体中至少有3条呈阳性，则该样本被归类为cin阳性。结果：63例标本中cin阳性32例（39.7%）。5年总体无病生存率为58.7%，cin阳性患者为46.9%，cin阴性患者为71.0% I风险比（HR）， 2.34； 95%可信区间（CI）， 1.04-5.26；P = 0.04]。5年总生存率为81.0%，cin阳性患者为68.7%，cin阴性患者为93.5% （HR, 5.64; 95% CI, 1.23-25.70; p = 0.026）。在调整病理分期、肿瘤分期、性别、年龄、吸烟史等因素后的多因素分析中，与cin阴性患者相比，cin阳性状态仍与总生存率降低显著相关（HR, 8.48: 95% CI, 1.66-43.42; p 0.010）。结论：FISH分析可有效检测肺原发性AC的CIN。CIN与AC预后不良相关，因此可作为该疾病的独立预后因素。2008爱思唯尔爱尔兰有限公司版权所有。
Background: in this study, we Sought to evaluate the prognostic importance of chromosomal instability (CIN) in adenocarcinoma (AC) of the lung. The relationship between CIN detected by fluorescence in situ hybridization (FISH) and survival in AC patients was examined.Methods: Sixty-three Surgical specimens Of lung AC were analyzed. To identify tumors with CIN, p 16 and multi-target DNA FISH assays for c-myc, chromosome 6, EGFR, and chromosome 5 (LAVysion, Vysis) were performed on nuclei extracted from paraffin-embedded tumor tissues. Survival rates were compared in terms of sex, age, histology, T factor, N factor, CIN, and smoking Status. A sample was classified as CIN-positive if at least three of the five chromosomes were positive.Results: Out of the 63 specimens, 32 (39.7%) were CIN-positive. The 5-year overall disease-free survival rate was 58.7% as a whole, 46.9% for CIN-positive patients and 71.0% for the CIN-negative patients I hazard ratio (HR), 2.34: 95% confidence interval (CI), 1.04-5.26; p = 0.04]. The 5-year overall survival rate was 81.0%, 68.7% for CIN-positive patients and 93.5% for the CIN-negative patients (HR, 5.64; 95% CI, 1.23-25.70; p = 0.026). In multivariate analysis after adjusting for pathologic nodal staging, tumor staging, sex, age, and smoking history, compared with the CIN-negative patients, the CIN-positive Status remained significantly associated with decreased overall survival (HR, 8.48: 95% CI, 1.66-43.42; p 0.010).Conclusions: CIN can be effectively detected in primary AC of lung using FISH analysis. CIN is associated with poor Prognosis for AC, and may thus be utilized as an independent Prognostic factor for the disease. (C) 2008 Elsevier Ireland Ltd. All rights reserved.