EFFECTS OF HUMAN AND SALMON-CALCITONIN ON HUMAN ARTICULAR CHONDROCYTES CULTIVATED IN CLUSTERS
EFFECTS OF HUMAN AND SALMON-CALCITONIN ON HUMAN ARTICULAR CHONDROCYTES CULTIVATED IN CLUSTERS
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DOI：
10.1210/jcem-69-2-259
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发表时间：
1989-08-01
影响因子：
5.8
通讯作者：
BASSLEER, R
中科院分区：
文献类型：
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作者：
FRANCHIMONT, P;BASSLEER, C;BASSLEER, R
在三维软骨细胞中研究了 20 天期间不同药理学浓度（0、5、10、100 和 1000 ng/mL）的合成人降钙素 (hCT) 和鲑鱼降钙素 (sCT) 对人关节软骨细胞掺入 [3H] 胸苷以及产生蛋白聚糖 (PG) 和 II 型胶原 (coll II) 的影响文化模型。 [3H]在软骨细胞簇中测量胸苷摄取，并且每 4 天对培养基和细胞聚集体进行特定的 PG 和 coll II RIA；通过添加培养基及其相应簇中发现的量，还评估了不同培养持续时间的总 PG 和 coll II 产量。无论剂量如何，与 hCT 或 sCT 一起孵育均不会影响 [3H] 胸苷摄取。对于每个培养周期，PG 和 coll II 释放到培养基中、簇含量和总产量均以剂量依赖性方式显着增加。这些参数的累积曲线显示，在 hCT 和 sCT 剂量为 0、5 和 10 ng/mL 时，随着培养时间的延长，这些参数逐渐显着增加。使用 10、100 和 1000 ng/mL 获得的累积曲线很少彼此存在显着差异。 hCT 或 sCT 的使用没有差异。因此，CT 对三维培养中的人关节软骨细胞没有增殖作用，但对 PG 和 coll II 的产生显示出剂量依赖性和长期的刺激作用。除了其他已知的作用外，CT 还可能具有软骨保护特性。
The effects of different pharmacological concentrations (0, 5, 10, 100, and 1000 ng/mL) of synthetic human calcitonin (hCT) and salmon calcitonin (sCT) on the incorporation of [3H]thymidine and production of proteoglycans (PG) and type II collagen (coll II) by human articular chondrocytes during a 20-day period were studied in a tridimensional chondrocyte culture model. [3H]Thymidine uptake was measured in chondrocyte clusters, and specific PG and coll II RIAs were performed every 4 days on the culture medium and cell aggregates; total PG and coll II production were also assessed at different culture durations by adding the amounts found in culture media and their corresponding clusters. Incubation with hCT or sCT did not affect [3H]thymidine uptake regardless of the dose. For each culture period, PG and coll II release into culture medium, cluster content, and total production increased significantly in a dose-dependent manner. Cumulative curves for these parameters showed a progressive significant increase with culture duration at hCT and sCT doses of 0, 5, and 10 ng/mL. Cumulative curves obtained with 10, 100, and 1000 ng/mL were seldom significantly different from one another. No differences emerged between the use of hCT or sCT. Thus, CT exerted no proliferative effect on human articular chondrocytes in tridimensional culture, but displayed a dose-dependent and prolonged stimulatory effect on PG and coll II production. CT may possess chondroprotective properties in addition to its other known effects.